Linked Life Data

8386426

Source:http://linkedlifedata.com/resource/pubmed/id/8386426

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1993-5-17
pubmed:abstractText
Hydrolysis of inositol phospholipids caused by muscarinic agonists was studied in the guinea-pig parotid gland (PG) and cerebral cortex (CX). The present study describes the effect of two muscarinic agonists, carbachol and oxotremorine, on stimulation of phosphoinositide hydrolysis and on binding of [3H]NMS in the presence of the irreversible muscarinic antagonist benzilyl choline mustard (BCM). Carbachol and oxotremorine stimulated the formation of inositol phosphates in PG, pD2(Carb) = 5.3 +/- 0.1, pD2(Oxo) = 5.9 +/- 0.1 and in CX, pD2(Carb) = 4.3 +/- 0.2, pD2(Oxo) = 5.8 +/- 0.2. In the present study slices from both tissues have been exposed to 0.1 microM BCM for 2, 5 and 10 min. Treatment for 10 min caused a 75-85% reduction in specific [3H]N-methyl scopolamine ([3H]NMS) binding sites in both PG and CX. Following 2 min BCM treatment of PG a marked decrease in pD2 value of the carbachol-stimulated inositol phosphate formation was found. This effect was not found in CX. The results showed that a 30-40% reduction in binding sites shifted the carbachol concentration response curve to the right by one order of magnitude and reduced the oxotremorine-induced release of inositol phosphates by approximately 20%. In PG, the BCM-induced reduction of the carbachol-stimulated inositol phosphate formation was paralleled by the reduction in receptor binding sites. Similar treatment, but in CX, showed a lower reduction of the carbachol-stimulated inositol phosphate formation as compared to the reduction in receptor-binding sites.(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0001-6772
pubmed:author
pubmed:issnType
Print
pubmed:volume
147
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
289-95
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Studies of muscarinic receptor reserve linked to phosphoinositide hydrolysis in parotid gland and cerebral cortex.
pubmed:affiliation
Department of Physiology, University of Gothenburg, Sweden.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't