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rdf:type |
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lifeskim:mentions |
umls-concept:C0007634,
umls-concept:C0021549,
umls-concept:C0025914,
umls-concept:C0026809,
umls-concept:C0030685,
umls-concept:C0051926,
umls-concept:C0205102,
umls-concept:C0391871,
umls-concept:C0596235,
umls-concept:C0680255,
umls-concept:C0851285,
umls-concept:C1283071,
umls-concept:C1314939,
umls-concept:C1622418,
umls-concept:C1698986,
umls-concept:C1963578
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pubmed:issue |
10
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pubmed:dateCreated |
1993-5-5
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pubmed:abstractText |
Mouse T-lymphoma cells contain a unique type of internal vesicle which bands at the relatively light density of 1.07 g/cc. These vesicles do not contain any detectable Golgi, endoplasmic reticulum, plasma membrane, or lysosomal marker protein activities. Binding of [3H]inositol 1,4,5-trisphosphate (IP3) to these internal vesicles reveals the presence of a single, high affinity class of IP3 receptor with a dissociation constant (Kd) of 1.6 +/- 0.3 nM. Using a panel of monoclonal and polyclonal antibodies against IP3 receptor, we have established that the IP3 receptor (approximately 260 kDa) displays immunological cross-reactivity with the rat brain IP3 receptor. Polymerase chain reaction analysis of first-strand cDNAs from both mouse T-lymphoma cells and rat brain tissues reveals that the IP3 receptor transcript in mouse T-lymphoma cells belongs to the short form (non-neuronal form) and not the long form (neuronal form) detected in rat brain tissue. Scatchard plot analysis shows that high affinity binding occurs between ankyrin and the IP3 receptor with a Kd of 0.2 nM. Most importantly, the binding of ankyrin to the light density vesicles significantly inhibits IP3 binding and IP3-induced internal Ca2+ release. These findings suggest that the cytoskeleton plays a pivotal role in the regulation of IP3 receptor-mediated internal Ca2+ release during lymphocyte activation.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Ankyrins,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Channels,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Single-Stranded,
http://linkedlifedata.com/resource/pubmed/chemical/ITPR1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Inositol 1,4,5-Trisphosphate...,
http://linkedlifedata.com/resource/pubmed/chemical/Inositol Phosphates,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cytoplasmic and Nuclear
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
5
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pubmed:volume |
268
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7290-7
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:8385102-Animals,
pubmed-meshheading:8385102-Ankyrins,
pubmed-meshheading:8385102-Base Sequence,
pubmed-meshheading:8385102-Calcium,
pubmed-meshheading:8385102-Calcium Channels,
pubmed-meshheading:8385102-Cytoskeleton,
pubmed-meshheading:8385102-DNA, Single-Stranded,
pubmed-meshheading:8385102-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:8385102-Fluorescent Antibody Technique,
pubmed-meshheading:8385102-Humans,
pubmed-meshheading:8385102-Inositol 1,4,5-Trisphosphate Receptors,
pubmed-meshheading:8385102-Inositol Phosphates,
pubmed-meshheading:8385102-Lipid Metabolism,
pubmed-meshheading:8385102-Lymphoma, T-Cell,
pubmed-meshheading:8385102-Mice,
pubmed-meshheading:8385102-Molecular Sequence Data,
pubmed-meshheading:8385102-Receptors, Cell Surface,
pubmed-meshheading:8385102-Receptors, Cytoplasmic and Nuclear,
pubmed-meshheading:8385102-Transcription, Genetic,
pubmed-meshheading:8385102-Tumor Cells, Cultured
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pubmed:year |
1993
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pubmed:articleTitle |
The involvement of ankyrin in the regulation of inositol 1,4,5-trisphosphate receptor-mediated internal Ca2+ release from Ca2+ storage vesicles in mouse T-lymphoma cells.
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pubmed:affiliation |
Department of Cell Biology and Anatomy, School of Medicine, University of Miami, Florida 33101.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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