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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1993-10-20
|
| pubmed:abstractText |
We studied the influence of diet during diarrhea on gut mucosal barrier in a suckling rat model. Rat pups were inoculated with IDIR virus (a group B rotavirus) at 10 d of age. Beginning 2 d postinfection, in addition to maternal milk, group CM received a daily gavage of cow milk and group GG received Lactobacillus casei strain GG, a human strain previously shown to survive the passage through the gastrointestinal tract and temporarily colonize the gut. Group CMGG received a combination of these, and control animals were gavaged with tap water. At 21 d of age, jejunal absorption of intact and degraded horseradish peroxidase (HRP) in Ussing chamber was markedly higher in IDIR virus-infected than in noninfected controls. In the two groups gavaged with cow milk, group CM and group CMGG, the numbers of specific antibody-secreting cells (enumerated by the solid-phase enzyme-linked immunospot assay) against beta-lactoglobulin were significantly higher than in the groups that had not received cow milk. In parallel with immune system activation, a statistically significant increase in the absorption of intact HRP (mean and 95% confidence interval, ng x h-1 x cm-2) was detected: group CM, 302 (155, 586); group CMGG, 174 (56, 545); infected controls, 121 (57, 257); and group GG, 44 (8, 254). A decrease in the uptake of intact HRP (F = 3.64, p = 0.06) and degraded HRP (F = 9.50, p = 0.004) was associated with the introduction of L. casei GG to the diet, irrespective of coexposure to cow milk.(ABSTRACT TRUNCATED AT 250 WORDS)
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0031-3998
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
33
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
548-53
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8378109-Animals,
pubmed-meshheading:8378109-Animals, Suckling,
pubmed-meshheading:8378109-Antibody-Producing Cells,
pubmed-meshheading:8378109-Diet,
pubmed-meshheading:8378109-Female,
pubmed-meshheading:8378109-Food Microbiology,
pubmed-meshheading:8378109-Gastroenteritis,
pubmed-meshheading:8378109-Horseradish Peroxidase,
pubmed-meshheading:8378109-Intestinal Mucosa,
pubmed-meshheading:8378109-Jejunum,
pubmed-meshheading:8378109-Lactobacillus casei,
pubmed-meshheading:8378109-Lactoglobulins,
pubmed-meshheading:8378109-Male,
pubmed-meshheading:8378109-Microscopy, Electron,
pubmed-meshheading:8378109-Permeability,
pubmed-meshheading:8378109-Rats,
pubmed-meshheading:8378109-Rats, Wistar,
pubmed-meshheading:8378109-Rotavirus Infections
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
Diet during rotavirus enteritis affects jejunal permeability to macromolecules in suckling rats.
|
| pubmed:affiliation |
University of Tampere, Department of Clinical Sciences, Finland.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|