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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:dateCreated |
1993-10-18
|
| pubmed:databankReference | |
| pubmed:abstractText |
Nucleotide sequencing of the fusion protein (F) gene of phocid distemper virus-2 (PDV-2), recently isolated from Baikal seals (Phoca sibirica), revealed an open reading frame (nucleotides 84 to 2075) with two potential in-frame ATG translation initiation codons. We suggest that the second in-frame ATG triplet at positions 264 to 266 initiates the translation, resulting in a protein of 537 amino acid residues with a calculated M(r) of 63,035. The putative F1/F2 cleavage site, located approximately 100 amino acid residues from the N terminus, is identical to those of the F proteins of phocid distemper virus-1 (PDV-1) isolated from European harbour seals (Phoca vitulina) and of canine distemper virus (CDV). A full scale comparison of morbillivirus F genes reveals that the conserved F0 extracellular protein-encoding region contains a large number of non-expressed mutations, suggesting that this part of the protein is under strong functional constraints. Phylogenetic analysis of morbillivirus F gene nucleotide sequences revealed a closer evolutionary relationship between PDV-2 and CDV than between PDV-1 and PDV-2. These data were supported by cross-reactivity patterns of PDV-2 and CDV obtained with monoclonal antibodies to structural proteins of PDV-1 and CDV, and suggest that PDV-2 is a strain of CDV, resulting from a trans-species infection.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Codon,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/F protein, Phocine distemper virus,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Fusion Proteins
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| pubmed:status |
MEDLINE
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| pubmed:month |
Sep
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| pubmed:issn |
0022-1317
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
74 ( Pt 9)
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1989-94
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| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:8376973-Amino Acid Sequence,
pubmed-meshheading:8376973-Animals,
pubmed-meshheading:8376973-Base Sequence,
pubmed-meshheading:8376973-Codon,
pubmed-meshheading:8376973-DNA, Viral,
pubmed-meshheading:8376973-Distemper Virus, Canine,
pubmed-meshheading:8376973-Genes, Viral,
pubmed-meshheading:8376973-Measles virus,
pubmed-meshheading:8376973-Molecular Sequence Data,
pubmed-meshheading:8376973-Molecular Weight,
pubmed-meshheading:8376973-Paramyxoviridae,
pubmed-meshheading:8376973-Phylogeny,
pubmed-meshheading:8376973-RNA, Messenger,
pubmed-meshheading:8376973-Seals, Earless,
pubmed-meshheading:8376973-Sequence Homology, Amino Acid,
pubmed-meshheading:8376973-Sequence Homology, Nucleic Acid,
pubmed-meshheading:8376973-Viral Fusion Proteins
|
| pubmed:year |
1993
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| pubmed:articleTitle |
Fusion protein gene nucleotide sequence similarities, shared antigenic sites and phylogenetic analysis suggest that phocid distemper virus type 2 and canine distemper virus belong to the same virus entity.
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| pubmed:affiliation |
Seal Rehabilitation and Research Centre, Pieterburen, The Netherlands.
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| pubmed:publicationType |
Journal Article
|