|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
1
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pubmed:dateCreated |
1993-10-8
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|
pubmed:abstractText |
For the structure determination of symmetric protein dimers it is necessary to distinguish between intra- and inter-subunit NOEs. A method is presented to measure selectively the inter-subunit NOEs using uniform 15N and 13C isotope labelling. This is accomplished by double filtered 2D NOE experiments on mixtures of native protein with isotope-labeled protein. The method has been applied to the Arc repressor and allows the characterization of virtually all proton-proton NOEs in terms of their intra- or inter-subunit nature.
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pubmed:language |
eng
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pubmed:journal |
|
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pubmed:citationSubset |
IM
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pubmed:chemical |
|
|
pubmed:status |
MEDLINE
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pubmed:month |
Sep
|
|
pubmed:issn |
0014-5793
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|
pubmed:author |
|
|
pubmed:issnType |
Print
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|
pubmed:day |
6
|
|
pubmed:volume |
330
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
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pubmed:pagination |
105-9
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|
pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
|
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pubmed:year |
1993
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|
pubmed:articleTitle |
Observation of inter-subunit nuclear Overhauser effects in a dimeric protein. Application to the Arc repressor.
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|
pubmed:affiliation |
Bijvoet Center for Biomolecular Research, Utrecht, The Netherlands.
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|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|
