Linked Life Data

8358837

Source:http://linkedlifedata.com/resource/pubmed/id/8358837

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
1993-9-27
pubmed:abstractText
A fragment of cDNA containing the xylanase gene (XLN) of Cryptococcus albidus was inserted into the vector pJHS which contains the selectable marker his3. To permit expression of the XLN gene, the xylose reductase promoter XYL1 from Pichia stipitis was introduced in front of the XLN gene. P. stipitis PJH53, a histidine-dependent strain, transformed with this plasmid produced an active extracellular xylanase. The enzyme secreted by P. stipitis had an apparent molecular mass of 50 kDa, which was slightly greater than that of the native 48-kDa xylanase produced by C. albidus. The expression of the XLN gene in P. stipitis allowed it to use xylan as a carbon source and to ferment xylan directly to ethanol under anaerobic conditions.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0172-8083
pubmed:author
pubmed:issnType
Print
pubmed:volume
24
pubmed:geneSymbol
XLN
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
94-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:articleTitle
Secretion of a Cryptococcus albidus xylanase in Pichia stipitis resulting in a xylan fermenting transformant.
pubmed:affiliation
Centre de Recherche en Microbiologie Appliquée, Institut Armand-Frappier, Québec, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't