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rdf:type |
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lifeskim:mentions |
umls-concept:C0010453,
umls-concept:C0030520,
umls-concept:C0034693,
umls-concept:C0034721,
umls-concept:C0205107,
umls-concept:C0205225,
umls-concept:C0458083,
umls-concept:C0552639,
umls-concept:C1159573,
umls-concept:C1280500,
umls-concept:C1709694
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pubmed:dateCreated |
1993-9-1
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pubmed:abstractText |
The development of satisfactory cell culture models for the study of parathyroid hormone (PTH)-induced inhibition of Pi transport has proven difficult. Using subcellular fractionation techniques we investigated the response of primary cultures of rat proximal tubular cells to PTH-(1-34). Specific binding of 125I-bPTH-(1-34) occurred at 2 degrees C. After 5 min of rewarming, trypsin-releasable radioactivity decreased from 90 to 50%, indicating internalization of the ligand. Cell disruption, followed by density centrifugation with 17% Percoll either directly after binding at 2 degrees C or post-rewarming for 20 min, showed a shift of 125I label from the plasma membrane (5'-nucleotidase) to lysosomal fractions (beta-D-glucosaminidase), confirming the sequential occurrence of cell surface binding, internalization and transport to lysosomes of 125I-bPTH-(1-34). Reculture at 37 degrees C revealed steady accumulation of trichloroacetic acid-soluble radioactivity in the medium, indicating degradation of 125I-bPTH-(1-34). Phosphate transport in the absence of sodium was minimal. Incubation of the cells with bPTH-(1-34) resulted in up to 50% inhibition of sodium-dependent phosphate transport. Prior phosphate depletion abrogated the response to PTH.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8343117-1063404,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8343117-1141439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8343117-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8343117-2155534,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8343117-2419328,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8343117-2548989,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8343117-2841095,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8343117-4061629,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8343117-5016649,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8343117-566717,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8343117-6119031,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8343117-6292232,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8343117-6292894,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8343117-6321147,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8343117-828141,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8343117-942051
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
293 ( Pt 2)
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
377-80
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8343117-Animals,
pubmed-meshheading:8343117-Binding Sites,
pubmed-meshheading:8343117-Biological Transport,
pubmed-meshheading:8343117-Cell Fractionation,
pubmed-meshheading:8343117-Cells, Cultured,
pubmed-meshheading:8343117-Kidney Tubules, Proximal,
pubmed-meshheading:8343117-Parathyroid Hormone,
pubmed-meshheading:8343117-Peptide Fragments,
pubmed-meshheading:8343117-Phosphates,
pubmed-meshheading:8343117-Rats
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pubmed:year |
1993
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pubmed:articleTitle |
Parathyroid hormone transport effects and hormonal processing in primary cultured rat proximal tubular cells.
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pubmed:affiliation |
Department of Medicine, Tulane University Medical Center, New Orleans, LA 70112.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.
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