Linked Life Data

8330623

Source:http://linkedlifedata.com/resource/pubmed/id/8330623

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1993-8-19
pubmed:abstractText
During maturation of reticulocytes to erythrocytes, small vesicles, termed exosomes, are released into the extracellular medium. GTP-binding proteins associated with exosomes were identified by separating proteins by sodium dodecyl sulfate polyacrylamide gel electrophoresis, transferring the proteins to nitrocellulose, and probing the blots with [alpha-32P]GTP. At least three GTP-binding proteins were detected with a molecular mass of 27, 26, and 20 kDa. Binding of GTP by exosomes was resistant to trypsin in the absence, but not in the presence of detergent. This indicates that the GTP-binding proteins are within the lumen of exosomes. During reticulocyte maturation, the amount of GTP-binding proteins released from reticulocytes was proportional to the amount of exosomes released. Western blot analysis demonstrated that the 27 kDa, 26 kDa, and 20 kDa proteins were Rab5p, Rab4p and ADP-ribosylation factor (ARF), respectively. In reticulocytes, Rab4p was highly enriched in exosomes and endosomes compared to plasma membrane and cytosol. Although mainly cytosolic, ARF was also found associated with endosomes and exosomes but not with plasma membrane. In contrast to Rab4p and ARF, Rab5p was enriched in the plasma membrane compared to cytosol, exosomes and endosomes. As exosomes are believed to derive from endosomes, Rab4p and ARF may be involved in the mechanism of exosome formation.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0171-9335
pubmed:author
pubmed:issnType
Print
pubmed:volume
60
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
261-7
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
The small GTP-binding proteins Rab4 and ARF are associated with released exosomes during reticulocyte maturation.
pubmed:affiliation
URA 530 CNRS, Université des Sciences et Techniques du Languedoc, Montpellier/France.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't