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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
|
pubmed:dateCreated |
1993-8-19
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pubmed:abstractText |
We defined a hybridization condition for isolation of clones carrying long (dC-dA)n.(dG-dT)n microsatellites from a genomic plasmid library. By using (dC-dA)20 oligonucleotide as a probe and hybridizing under stringent conditions, more than two-thirds of the obtained plasmid carried the repeats that were longer than (dC-dA)14 and highly polymorphic.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Feb
|
pubmed:issn |
1050-3862
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pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
10
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pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
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pubmed:pagination |
2-5
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:8329220-Chromosomes, Human, Pair 11,
pubmed-meshheading:8329220-DNA, Satellite,
pubmed-meshheading:8329220-Hot Temperature,
pubmed-meshheading:8329220-Humans,
pubmed-meshheading:8329220-Nucleic Acid Hybridization,
pubmed-meshheading:8329220-Oligonucleotide Probes,
pubmed-meshheading:8329220-Polydeoxyribonucleotides,
pubmed-meshheading:8329220-Polymorphism, Genetic
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pubmed:year |
1993
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pubmed:articleTitle |
Selective isolation of highly polymorphic (dC-dA)n.(dG-dT)n microsatellites by stringent hybridization.
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pubmed:affiliation |
Oncogene Division, National Cancer Center Research Institute, Tokyo, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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