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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
14
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pubmed:dateCreated |
1993-8-10
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pubmed:abstractText |
We assayed the activity of the DNA repair protein O6-methylguanine-DNA methyltransferase (MGMT) in 60 human brain tumors to assess the effects of tumorigenesis in brain on DNA repair capability. Activity was not detectable (< 0.5 fmol/10(6) cells, i.e., < 300 molecules/cells) in 27% of the tumors. Measurable MGMT varied by more than 2 orders of magnitude, 0.5-104.1 fmol/10(6) cells. Mean tumor MGMT levels did not differ between the sexes but did vary widely between diagnostic groups. A significant inverse correlation was observed between tumor MGMT activity and patient age. We also assayed MGMT activity in overlying, histologically tumor-free brain resected with 25 tumors. Of these samples, 52% had no detectable MGMT activity, and the remainder had activity comparable to that in tumors ranging from 0.7-21.8 fmol/10(6) cells. MGMT activity in normal brain was also inversely correlated with patient age. For 15 of 25 (60%) paired samples, tumor activity was 2 to > 38-fold greater than that of normal brain; for 4 pairs (16%) tumor activity was 2.5 to > 17-fold lower than that of normal brain; the remaining 6 (24%) had no detectable activity in both tumor and normal tissue. These differences in the magnitudes and distributions of activities for tumor versus normal brain tissue were significant (P = 0.02), demonstrating that tumorigenesis in brain is often accompanied by marked elevation of MGMT.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0008-5472
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
53
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3416-20
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:8324751-Adolescent,
pubmed-meshheading:8324751-Adult,
pubmed-meshheading:8324751-Aged,
pubmed-meshheading:8324751-Astrocytoma,
pubmed-meshheading:8324751-Brain,
pubmed-meshheading:8324751-Brain Neoplasms,
pubmed-meshheading:8324751-Child,
pubmed-meshheading:8324751-Child, Preschool,
pubmed-meshheading:8324751-Ependymoma,
pubmed-meshheading:8324751-Female,
pubmed-meshheading:8324751-Glioma,
pubmed-meshheading:8324751-Humans,
pubmed-meshheading:8324751-Infant,
pubmed-meshheading:8324751-Male,
pubmed-meshheading:8324751-Medulloblastoma,
pubmed-meshheading:8324751-Methyltransferases,
pubmed-meshheading:8324751-Middle Aged,
pubmed-meshheading:8324751-O(6)-Methylguanine-DNA Methyltransferase,
pubmed-meshheading:8324751-Oligodendroglioma
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pubmed:year |
1993
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pubmed:articleTitle |
Comparison of O6-methylguanine-DNA methyltransferase activity in brain tumors and adjacent normal brain.
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pubmed:affiliation |
Department of Neurological Surgery, University of Washington, Seattle 98195.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.
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