Linked Life Data

8296385

Source:http://linkedlifedata.com/resource/pubmed/id/8296385

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1994-2-28
pubmed:abstractText
Fourteen Giardia duodenalis isolates were examined using the RAPD (random amplified polymorphic deoxyribonucleic acid) technique. Simple reproducible polymorphisms were generated using 3 different RAPD primers. The results generated by each primer were very similar and were significantly correlated with each other. These data were then compared to existing isoenzyme electrophoresis data on the same isolates. The RAPD data divided the isolates into 10 groupings or rapdemes while the isoenzyme data divided them into 10 similar zymodemes. Both methods grouped 4 isolates (BAH42, BAH44c9, BAH12c9 and BAH39c7), which comprised a phenotypically heterogeneous assemblage with respect to growth rate and metabolism, into similar groupings. The 2 methods were significantly correlated (P < 0.001). It will therefore be possible to use RAPD for the characterization of isolates of Giardia, and other parasites such as Cryptosporidium, which are refractory to cultivation in vitro.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0035-9203
pubmed:author
pubmed:issnType
Print
pubmed:volume
87
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
702-5
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:articleTitle
RAPD (random amplified polymorphic DNA) analysis of Giardia DNA and correlation with isoenzyme data.
pubmed:affiliation
Institute for Molecular Genetics and Animal Disease, School of Veterinary Studies, Murdoch University, Western Australia.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't