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pubmed-article:8291237pubmed:abstractTextCertain strains of Leishmania guyanensis carry persistently infecting double-stranded RNA viruses. The viral polymerase has been shown to have both transcriptase and replicase activity. To date, only full-length RNA transcription of minus and plus strands have been reported. This report describes the synthesis of a 320 nt transcript which is complementary to the 3' end of the minus strand RNA. This plus-stranded short transcript was first detected in an in vitro polymerase assay. It was also detected in virus-infected Leishmania cells by a reverse transcription-polymerase chain reaction assay and by Northern analysis of infected Leishmania cell RNA.lld:pubmed
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pubmed-article:8291237pubmed:articleTitleIdentification of a short viral transcript in Leishmania RNA virus-infected cells.lld:pubmed
pubmed-article:8291237pubmed:affiliationDepartment of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115.lld:pubmed
pubmed-article:8291237pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:8291237pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed
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