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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1994-2-24
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pubmed:abstractText |
Certain strains of Leishmania guyanensis carry persistently infecting double-stranded RNA viruses. The viral polymerase has been shown to have both transcriptase and replicase activity. To date, only full-length RNA transcription of minus and plus strands have been reported. This report describes the synthesis of a 320 nt transcript which is complementary to the 3' end of the minus strand RNA. This plus-stranded short transcript was first detected in an in vitro polymerase assay. It was also detected in virus-infected Leishmania cells by a reverse transcription-polymerase chain reaction assay and by Northern analysis of infected Leishmania cell RNA.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0042-6822
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
198
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
552-6
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:8291237-Animals,
pubmed-meshheading:8291237-Base Sequence,
pubmed-meshheading:8291237-Blotting, Northern,
pubmed-meshheading:8291237-DNA-Directed RNA Polymerases,
pubmed-meshheading:8291237-Leishmania guyanensis,
pubmed-meshheading:8291237-Molecular Sequence Data,
pubmed-meshheading:8291237-Polymerase Chain Reaction,
pubmed-meshheading:8291237-RNA, Double-Stranded,
pubmed-meshheading:8291237-RNA, Messenger,
pubmed-meshheading:8291237-RNA Viruses,
pubmed-meshheading:8291237-Transcription, Genetic
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pubmed:year |
1994
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pubmed:articleTitle |
Identification of a short viral transcript in Leishmania RNA virus-infected cells.
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pubmed:affiliation |
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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