Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1994-2-24
|
| pubmed:abstractText |
Three related clones encoding proteins (ATFa1, 2 and 3) with specific ATF/CRE DNA-binding activities have been isolated from HeLa cell cDNA libraries. All three isoforms have weak effects on the basal activity of the adenovirus E2a promoter. We present evidence suggesting that a C-terminal element of the ATFa molecules negatively interferes with the intrinsic activation function of these proteins. We also show that coexpression of ATFa with c-Jun, Jun-B or Jun-D stimulates ATFa-dependent reporter activity, while coexpression of c-Fos has no effect. Deletion analyses indicate that the metal-binding region of ATFa is dispensible for this effect, but that the domain comprising the leucine-zipper region of ATFa is required. Reciprocal co-immunoprecipitation experiments and electrophoretic band-shift assays with in vitro synthesized proteins reveal direct interactions between ATFa and Jun or Fos. The ATFa/c-Jun heterodimers, but not the ATFa/c-Fos complexes, bind efficiently to ATF, CRE or AP1 sites. The detection of ATFa-Jun complexes in crude extracts from HeLa cells transfected with ATFa and c-Jun expression vectors suggests that such ATFa/c-Jun heterodimers also form in vivo. Altogether these results indicate that the ATFa proteins may contribute to the modulation of the activity of the Jun/Fos complexes by altering their DNA-binding and transcriptional properties.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Activating Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Blood Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP Response...,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-fos,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-jun,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0950-9232
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
9
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
375-85
|
| pubmed:dateRevised |
2010-2-4
|
| pubmed:meshHeading |
pubmed-meshheading:8290251-Activating Transcription Factors,
pubmed-meshheading:8290251-Amino Acid Sequence,
pubmed-meshheading:8290251-Animals,
pubmed-meshheading:8290251-Base Sequence,
pubmed-meshheading:8290251-Blood Proteins,
pubmed-meshheading:8290251-Cell Line,
pubmed-meshheading:8290251-Cyclic AMP Response Element-Binding Protein,
pubmed-meshheading:8290251-DNA,
pubmed-meshheading:8290251-Genes, Reporter,
pubmed-meshheading:8290251-Genes, fos,
pubmed-meshheading:8290251-Genes, jun,
pubmed-meshheading:8290251-Genetic Vectors,
pubmed-meshheading:8290251-HeLa Cells,
pubmed-meshheading:8290251-Humans,
pubmed-meshheading:8290251-Isomerism,
pubmed-meshheading:8290251-Leucine Zippers,
pubmed-meshheading:8290251-Molecular Sequence Data,
pubmed-meshheading:8290251-Proto-Oncogene Proteins c-fos,
pubmed-meshheading:8290251-Proto-Oncogene Proteins c-jun,
pubmed-meshheading:8290251-Transcription, Genetic,
pubmed-meshheading:8290251-Transcription Factors,
pubmed-meshheading:8290251-Transfection
|
| pubmed:year |
1994
|
| pubmed:articleTitle |
Jun and Fos heterodimerize with ATFa, a member of the ATF/CREB family and modulate its transcriptional activity.
|
| pubmed:affiliation |
Laboratoire de Génétique Moléculaire des Eucaryotes (CNRS), Unité 184 (INSERM), Institut de Chimie Biologique, Strasbourg, France.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|