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rdf:type |
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lifeskim:mentions |
umls-concept:C0010453,
umls-concept:C0016693,
umls-concept:C0018561,
umls-concept:C0065555,
umls-concept:C0205225,
umls-concept:C0205332,
umls-concept:C0332120,
umls-concept:C0543482,
umls-concept:C0920487,
umls-concept:C1510411,
umls-concept:C1522492
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pubmed:issue |
1-2
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pubmed:dateCreated |
1994-2-24
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pubmed:abstractText |
Malachite green (MG) (green crystals with metallic luster and very soluble in water) is highly cytotoxic to mammalian cells and also acts as a liver tumor promoter. In view of its industrial importance and possible exposure to individuals, MG poses a potential environmental health hazard. We have studied the effect of MG on the formation of morphologically transformed colonies using Syrian hamster embryo (SHE) cell transformation assay. MG induced a dose-related increase in the formation of transformed foci, the optimum concentration being 0.05 micrograms/ml. Electron spin resonance (ESR) analysis using 5,5-dimethyl-1-pyrroline N-oxide (DMPO) as a spin-trapping agent showed the formation of reactive free radicals during the in vitro metabolism of MG. The present study suggests a close relationship between the morphological transformation of SHE cells by MG and the possible involvement of reactive free radical formation.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0304-3835
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
74
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
31-6
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:8287369-Animals,
pubmed-meshheading:8287369-Biotransformation,
pubmed-meshheading:8287369-Carcinogens,
pubmed-meshheading:8287369-Cell Line, Transformed,
pubmed-meshheading:8287369-Cell Transformation, Neoplastic,
pubmed-meshheading:8287369-Cells, Cultured,
pubmed-meshheading:8287369-Cricetinae,
pubmed-meshheading:8287369-Cyclic N-Oxides,
pubmed-meshheading:8287369-Dose-Response Relationship, Drug,
pubmed-meshheading:8287369-Electron Spin Resonance Spectroscopy,
pubmed-meshheading:8287369-Embryo, Mammalian,
pubmed-meshheading:8287369-Free Radicals,
pubmed-meshheading:8287369-Mesocricetus,
pubmed-meshheading:8287369-Mutagenicity Tests,
pubmed-meshheading:8287369-Mutagens,
pubmed-meshheading:8287369-Rosaniline Dyes,
pubmed-meshheading:8287369-Spin Labels
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pubmed:year |
1993
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pubmed:articleTitle |
Morphological transformation of Syrian hamster embryo cells in primary culture by malachite green correlates well with the evidence for formation of reactive free radicals.
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pubmed:affiliation |
Neuro OncologyDivision, Tata Memorial Centre, Parel, Bombay, India.
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pubmed:publicationType |
Journal Article
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