Linked Life Data

8284329

Source:http://linkedlifedata.com/resource/pubmed/id/8284329

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1994-2-14
pubmed:abstractText
Clinical photodynamic therapy (PDT) uses the photosensitizer photofrin II to produce singlet molecular oxygen and other reactive oxygen intermediates for localized tumor tissue cytotoxicity. In this report, we show that PDT enhances the DNA binding activity of nuclear factor kappa B (NF kappa B), a transactivator of cytokine gene expression. Photosensitization following a 16 h incubation of photofrin II induced NF kappa B binding activity in mouse leukemia L1210 cells 10-fold above that observed in exponentially growing cultures. Serum starvation, as well as drug-alone and light-alone controls, elevated basal NF kappa B binding activity two- to three-fold. Upstream stimulatory factor binding activity was not modulated by any of the cell treatments and was used to standardize gel mobility shift data. This study identifies porphyrin-mediated PDT as an inducer of NF kappa B binding activity, extending recent findings that NF kappa B activation is a general response to oxidative stress.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0031-8655
pubmed:author
pubmed:issnType
Print
pubmed:volume
58
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
753-6
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Nuclear factor kappa B binding activity in mouse L1210 cells following photofrin II-mediated photosensitization.
pubmed:affiliation
Department of Molecular Pharmacology & Toxicology, University of Southern California.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.