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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1994-2-14
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pubmed:abstractText |
The gene mutated in the human disease, X-linked agammaglobulinemia (XLA), is related to the Src gene family of cytoplasmic protein-tyrosine kinases and is designated Btk (Bruton's agammaglobulinemia tyrosine kinase; formerly Atk/Bpk; the human gene is denoted BTK, using capital letters according to the kinase nomenclature). We have recently reported that this gene is expressed in B lymphocytes and that the specific mRNA was undetectable in T cells using Northern blotting. Further analyses of different sources of B and T lymphocytes confirmed this pattern. However, BTK transcripts were undetectable in four plasmacytoma lines. Moreover, as virtually normal amounts of BTK transcripts were found in PBMC from two patients carrying a point mutation in BTK, despite low B cell numbers, we anticipated that the gene would also be expressed in cells of other lineages. The erythroleukemia cell line K-562, the promyelocytic line HL-60 and the histiocytic lymphoma line U-937 were found to have BTK mRNA levels comparable to B cells. BTK mRNA was also detected in monocytes from healthy donors as well as in the human immature basophilic cell line KU812, in the human mast cell leukemia cell line HMC-1 and in the CD34 expressing myeloblast KG-1. A similar expression pattern was obtained when BTK protein was analyzed by immunoprecipitation and Western blotting. Using a polymerase chain reaction-based analysis, a small amount (less than 1% of the level in B cells) of BTK mRNA was identified in T lymphocytes. Our findings are compatible with a general expression of the BTK gene in hematopoietic cells, except in T lymphocytes and plasma cells, in which the transcript level is selectively down-regulated.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/Phorbol Esters,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Tretinoin
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0022-1767
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
152
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pubmed:owner |
NLM
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pubmed:authorsComplete |
N
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pubmed:pagination |
557-65
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:8283037-Agammaglobulinemia,
pubmed-meshheading:8283037-B-Lymphocytes,
pubmed-meshheading:8283037-Base Sequence,
pubmed-meshheading:8283037-Bone Marrow,
pubmed-meshheading:8283037-Cell Differentiation,
pubmed-meshheading:8283037-DNA Primers,
pubmed-meshheading:8283037-Gene Expression,
pubmed-meshheading:8283037-Humans,
pubmed-meshheading:8283037-Mast Cells,
pubmed-meshheading:8283037-Molecular Sequence Data,
pubmed-meshheading:8283037-Phorbol Esters,
pubmed-meshheading:8283037-Plasma Cells,
pubmed-meshheading:8283037-Protein-Tyrosine Kinases,
pubmed-meshheading:8283037-RNA, Messenger,
pubmed-meshheading:8283037-T-Lymphocytes,
pubmed-meshheading:8283037-Tretinoin,
pubmed-meshheading:8283037-X Chromosome
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pubmed:year |
1994
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pubmed:articleTitle |
Expression of Bruton's agammaglobulinemia tyrosine kinase gene, BTK, is selectively down-regulated in T lymphocytes and plasma cells.
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pubmed:affiliation |
Center for BioTechnology, Karolinska Institute, NOVUM, Huddinge, Sweden.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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