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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1994-2-10
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pubmed:databankReference |
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pubmed:abstractText |
Prostacyclin synthase (PGIS) from bovine aorta was purified using conventional purification procedures including detergent solubilization, Sephacryl S-300 gel filtration and Mono Q high performance liquid chromatography. Polyclonal antiserum raised against the 52-kDa protein bound specifically to PGIS. After immobilization to Protein A-sepharose, the PGIS-antibody complex displayed PGIS activity. Results based on (1) elution profile of the Mono Q column, (2) two-dimensional gel electrophoresis and (3) N-terminal amino acid sequence suggested that PGIS is heterogenous. Amino acid sequences of N-terminus and a tryptic peptide led us to isolate a partial cDNA fragment.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cytochrome P-450 Enzyme System,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/Detergents,
http://linkedlifedata.com/resource/pubmed/chemical/Intramolecular Oxidoreductases,
http://linkedlifedata.com/resource/pubmed/chemical/Isomerases,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Prostaglandin H2,
http://linkedlifedata.com/resource/pubmed/chemical/Prostaglandins H,
http://linkedlifedata.com/resource/pubmed/chemical/Trypsin,
http://linkedlifedata.com/resource/pubmed/chemical/prostacyclin synthetase
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
30
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pubmed:volume |
197
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1041-8
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:8280118-Amino Acid Sequence,
pubmed-meshheading:8280118-Animals,
pubmed-meshheading:8280118-Aorta,
pubmed-meshheading:8280118-Base Sequence,
pubmed-meshheading:8280118-Cattle,
pubmed-meshheading:8280118-Chromatography, Affinity,
pubmed-meshheading:8280118-Chromatography, Gel,
pubmed-meshheading:8280118-Chromatography, Liquid,
pubmed-meshheading:8280118-Cytochrome P-450 Enzyme System,
pubmed-meshheading:8280118-DNA, Complementary,
pubmed-meshheading:8280118-DNA Primers,
pubmed-meshheading:8280118-Detergents,
pubmed-meshheading:8280118-Electrophoresis, Gel, Two-Dimensional,
pubmed-meshheading:8280118-Intramolecular Oxidoreductases,
pubmed-meshheading:8280118-Isomerases,
pubmed-meshheading:8280118-Kinetics,
pubmed-meshheading:8280118-Male,
pubmed-meshheading:8280118-Microsomes,
pubmed-meshheading:8280118-Molecular Sequence Data,
pubmed-meshheading:8280118-Molecular Weight,
pubmed-meshheading:8280118-Muscle, Smooth, Vascular,
pubmed-meshheading:8280118-Peptide Fragments,
pubmed-meshheading:8280118-Polymerase Chain Reaction,
pubmed-meshheading:8280118-Prostaglandin H2,
pubmed-meshheading:8280118-Prostaglandins H,
pubmed-meshheading:8280118-Seminal Vesicles,
pubmed-meshheading:8280118-Sheep,
pubmed-meshheading:8280118-Trypsin
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pubmed:year |
1993
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pubmed:articleTitle |
Bovine prostacyclin synthase: purification and isolation of partial cDNA.
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pubmed:affiliation |
Department of Internal Medicine, University of Texas-Houston 77030.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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