Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1994-1-31
pubmed:abstractText
Divalent cation (Ca2+ and Mg2+) and hydrogen ion-induced fusions of small unilamellar phosphatidic acid vesicles were studied by the use of fluorescence fusion assay. These fusogenic ions also increased interfacial tension and reduced the surface dielectric constant of phosphatidic acid membranes as the ion concentration increased. A good correlation was found between the threshold of vesicle fusion and the degree of changes in such membrane properties induced by these fusogenic ions. At the fusion threshold point, the increase in interfacial tension and the decreased surface dielectric constant of the membrane were approximately 6 and 14 dyn/cm, respectively, regardless of Ca2+, Mg2+ or H+. Such changes correspond to the increases in surface hydrophobicity of the membrane. As these fusogenic ionic concentrations increased, the electrophoretic mobility of the phosphatidic acid vesicle decreased, because of the binding of ions to negatively charged sites of the membranes. However, the quantities of the reduced negative surface charges were not necessarily in good correlation with the threshold of vesicle fusion. It is suggested that the complexes of the phosphate group with these fusogenic ions are responsible for increases in the surface hydrophobicity of the membrane, which is an important factor for membrane fusion.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0009-3084
pubmed:author
pubmed:issnType
Print
pubmed:volume
65
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
193-204
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Ion-induced fusion of phosphatidic acid vesicles and correlation between surface hydrophobicity and membrane fusion.
pubmed:affiliation
Department of Biophysical Sciences, State University of New York at Buffalo 14214.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.