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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1994-2-3
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pubmed:databankReference |
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pubmed:abstractText |
The neurotoxin gene from Clostridium botulinum type G was cloned as a series of overlapping DNA fragments generated using polymerase chain reaction (PCR) technology and primers designed to conserved regions of published botulinal toxin (BoNT) sequences. The 5'-end of the gene was obtained using a primer based on a conserved region of the nontoxic-nonhaemagglutinin gene lying upstream of the toxin gene. Translation of the nucleotide sequence derived from the cloned PCR fragments demonstrated that the gene encodes a protein of 1297 amino acid residues (rmm 149, 147). Comparative alignment of the determined BoNT/G sequence with those of other clostridial neurotoxins revealed highest sequence relatedness (approx. 58% amino acid identity) with BoNT/B of proteolytic and non-proteolytic C. botulinum. Tetanus toxin (TeTx) and other BoNT types revealed lower levels of relatedness with BoNT/G (approximate range 35-42% amino acid identity).
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0006-3002
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
14
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pubmed:volume |
1216
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
487-91
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:8268233-Amino Acid Sequence,
pubmed-meshheading:8268233-Base Sequence,
pubmed-meshheading:8268233-Botulinum Toxins,
pubmed-meshheading:8268233-Cloning, Molecular,
pubmed-meshheading:8268233-Clostridium botulinum,
pubmed-meshheading:8268233-DNA, Bacterial,
pubmed-meshheading:8268233-DNA Primers,
pubmed-meshheading:8268233-Genes, Bacterial,
pubmed-meshheading:8268233-Molecular Sequence Data,
pubmed-meshheading:8268233-Neurotoxins,
pubmed-meshheading:8268233-Phylogeny,
pubmed-meshheading:8268233-Polymerase Chain Reaction,
pubmed-meshheading:8268233-Sequence Homology, Amino Acid,
pubmed-meshheading:8268233-Toxoids
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pubmed:year |
1993
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pubmed:articleTitle |
Nucleotide sequence of the gene coding for Clostridium botulinum (Clostridium argentinense) type G neurotoxin: genealogical comparison with other clostridial neurotoxins.
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pubmed:affiliation |
Department of Microbiology, Institute of Food Research, Reading Laboratory, Earley Gate, UK.
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pubmed:publicationType |
Journal Article,
Comparative Study
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