rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
1994-1-26
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pubmed:abstractText |
The kinetics and signal transduction of inositol phosphate production were studied after a 20% stretch of neonatal rat cardiomyocytes in culture. Inositol trisphosphate (IP3) production was increased by 41% above control after 10-20 s of cellular stretch but returned to control after 120 s of stretch. The increase in IP3 was potentiated in high K+ medium and was inhibited by pertussis toxin, suggesting the existence of a pertussis toxin-sensitive G protein in signal transduction. Ion-pair HPLC analysis of cell extracts stretched for 20 s showed an increase in both IP3 isomers, mostly 1,4,5-IP3 (+66%) with a weak increase in IP4 (+10%), whereas 120 s stretch induced an increase in IP4 (+26% above control) associated with a decrease of 1,4,5-IP3 isomer as compared with 20 s stretch. It is concluded that the progressive increase in IP4 production associated with an early rise in IP3 after stretching myocardial cells may be a factor inducing the length-dependent activation of cardiac muscle through a modulation of intracellular free calcium concentration.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Inositol 1,4,5-Trisphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Inositol Phosphates,
http://linkedlifedata.com/resource/pubmed/chemical/Norepinephrine,
http://linkedlifedata.com/resource/pubmed/chemical/Pertussis Toxin,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium Chloride,
http://linkedlifedata.com/resource/pubmed/chemical/Type C Phospholipases,
http://linkedlifedata.com/resource/pubmed/chemical/Virulence Factors, Bordetella,
http://linkedlifedata.com/resource/pubmed/chemical/inositol 1,3,4-trisphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/inositol-1,3,4,5-tetrakisphosphate
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0022-2828
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
25
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
973-82
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:8263965-Animals,
pubmed-meshheading:8263965-Animals, Newborn,
pubmed-meshheading:8263965-Cells, Cultured,
pubmed-meshheading:8263965-GTP-Binding Proteins,
pubmed-meshheading:8263965-Heart,
pubmed-meshheading:8263965-Inositol 1,4,5-Trisphosphate,
pubmed-meshheading:8263965-Inositol Phosphates,
pubmed-meshheading:8263965-Kinetics,
pubmed-meshheading:8263965-Mechanoreceptors,
pubmed-meshheading:8263965-Myocardium,
pubmed-meshheading:8263965-Norepinephrine,
pubmed-meshheading:8263965-Pertussis Toxin,
pubmed-meshheading:8263965-Potassium Chloride,
pubmed-meshheading:8263965-Rats,
pubmed-meshheading:8263965-Rats, Wistar,
pubmed-meshheading:8263965-Signal Transduction,
pubmed-meshheading:8263965-Type C Phospholipases,
pubmed-meshheading:8263965-Virulence Factors, Bordetella
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pubmed:year |
1993
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pubmed:articleTitle |
Stretch-induced inositol trisphosphate and tetrakisphosphate production in rat cardiomyocytes.
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pubmed:affiliation |
Unité INSERM U.2, Faculté de Médecine, Creteil, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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