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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
36
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pubmed:dateCreated |
1994-1-27
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pubmed:abstractText |
To analyze the mechanism of the cell type-specific expression of rat angiotensin II type 1a receptor (AT1a-R) gene, we isolated the 5'-portion of the gene and identified multiple positive and negative regulatory sequences that regulate its transcription. Primer extension and S1 mapping identified a transcriptional initiation site at 33 (position +1) base pairs (bp) downstream of TATA sequence. The transcriptional activities of various 5'-deletion mutants of the AT1a-R gene upstream region, fused to the chloramphenicol acetyltransferase (CAT) gene, were examined using rat vascular smooth muscle cells (A10) and glial cells expressing AT1a-R mRNA predominantly or PC12 cells expressing AT2-R and very small amounts of AT1a-R mRNA. A 980-bp 5'-flanking sequence contained at least three positive elements, P1 (-560 to -489), P2 (-331 to -201), and P3 (-201 to -61). P1 and P3 were active in the tested three cells, and P2 was functional only in glial and PC12 cells. In addition to these positive elements, there was negative element, N1 (-489 to -331), which was active only in PC12 cells. These elements, when cotransfected with the AT1a-CAT fusion gene, had competitive effects against its promoter activity. The present study suggests the presence of multiple trans-acting factors that act on these positive and negative cis-acting elements and regulate the cell type-specific expression of the rat AT1a-R gene.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Dec
|
pubmed:issn |
0021-9258
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pubmed:author | |
pubmed:issnType |
Print
|
pubmed:day |
25
|
pubmed:volume |
268
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pubmed:geneSymbol |
AT1aR
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
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pubmed:pagination |
26996-7003
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8262935-Animals,
pubmed-meshheading:8262935-Base Sequence,
pubmed-meshheading:8262935-Chloramphenicol O-Acetyltransferase,
pubmed-meshheading:8262935-DNA, Complementary,
pubmed-meshheading:8262935-Gene Expression Regulation,
pubmed-meshheading:8262935-Molecular Sequence Data,
pubmed-meshheading:8262935-Neuroglia,
pubmed-meshheading:8262935-PC12 Cells,
pubmed-meshheading:8262935-Rats,
pubmed-meshheading:8262935-Receptors, Angiotensin,
pubmed-meshheading:8262935-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:8262935-Transcription, Genetic,
pubmed-meshheading:8262935-Transfection
|
pubmed:year |
1993
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pubmed:articleTitle |
Regulatory elements that mediate expression of the gene for the angiotensin II type 1a receptor for the rat.
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pubmed:affiliation |
Second Department of Internal Medicine, Kansai Medical University, Osaka, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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