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pubmed-article:8262210pubmed:abstractTextAn antibody recognising acetylated core histones was used to immunoprecipitate chromatin fragments from proliferating human K562 cells and from cells induced to differentiate with the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA). The DNA of the acetylated chromatin was probed with sequences of platelet derived growth factor B chain (PDGF-B), a gene which is induced to strong expression upon differentiation. A high level of acetylation was observed before gene induction and no change seen following induction. This implies that core histone acetylation is an essential precondition for transcription.lld:pubmed
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pubmed-article:8262210pubmed:articleTitleHistone acetylation and gene induction in human cells.lld:pubmed
pubmed-article:8262210pubmed:affiliationBiophysics Laboratories, University of Portsmouth, UK.lld:pubmed
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