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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1994-1-26
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pubmed:abstractText |
An antibody recognising acetylated core histones was used to immunoprecipitate chromatin fragments from proliferating human K562 cells and from cells induced to differentiate with the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA). The DNA of the acetylated chromatin was probed with sequences of platelet derived growth factor B chain (PDGF-B), a gene which is induced to strong expression upon differentiation. A high level of acetylation was observed before gene induction and no change seen following induction. This implies that core histone acetylation is an essential precondition for transcription.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0014-5793
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
20
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pubmed:volume |
336
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pubmed:geneSymbol |
PDGF-B
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
23-6
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:8262210-Acetylation,
pubmed-meshheading:8262210-Cell Line,
pubmed-meshheading:8262210-Chromatin,
pubmed-meshheading:8262210-DNA,
pubmed-meshheading:8262210-Histones,
pubmed-meshheading:8262210-Humans,
pubmed-meshheading:8262210-Platelet-Derived Growth Factor,
pubmed-meshheading:8262210-Protein-Tyrosine Kinases,
pubmed-meshheading:8262210-Proto-Oncogene Proteins,
pubmed-meshheading:8262210-Proto-Oncogene Proteins c-sis,
pubmed-meshheading:8262210-Tetradecanoylphorbol Acetate
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pubmed:year |
1993
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pubmed:articleTitle |
Histone acetylation and gene induction in human cells.
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pubmed:affiliation |
Biophysics Laboratories, University of Portsmouth, UK.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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