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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
12
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pubmed:dateCreated |
1994-1-13
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pubmed:abstractText |
We have developed a direct enzyme immunoassay (EIA) for quantifying immunoreactive 11-keto-thromboxane B2 (iKTXB) in unprocessed human urine. Cross-reactivity with other thromboxane metabolites and prostanoids was negligible. Analytical recovery of 11-keto-TXB2 in urine specimens was 97.4% to 99.8%. Total imprecision for two clinical specimens was 8.5% and 12.2%. Intake of acetylsalicylic acid decreased the measured concentration of iKTXB. Cardiopulmonary bypass, a procedure known to activate platelets, increased the mean excretion rate of iKTXB 10-fold. Simultaneous gas chromatography-mass spectrometry analysis of 11-keto-TXB2 and 11-keto-2,3-dinor TXB2 in urine specimens (n = 17) from healthy subjects indicated that urinary iKTXB concentrations measured by EIA represented a sum of the two 11-keto metabolites. We conclude that the direct EIA is sufficiently sensitive, rapid, simple, and specific to allow screening for alterations in thromboxane biosynthesis in patients.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0009-9147
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
39
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2470-7
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8252718-Aspirin,
pubmed-meshheading:8252718-Cardiopulmonary Bypass,
pubmed-meshheading:8252718-False Negative Reactions,
pubmed-meshheading:8252718-Gas Chromatography-Mass Spectrometry,
pubmed-meshheading:8252718-Humans,
pubmed-meshheading:8252718-Hydrogen-Ion Concentration,
pubmed-meshheading:8252718-Immunoenzyme Techniques,
pubmed-meshheading:8252718-Platelet Activation,
pubmed-meshheading:8252718-Quality Control,
pubmed-meshheading:8252718-Sensitivity and Specificity,
pubmed-meshheading:8252718-Thromboxane B2
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pubmed:year |
1993
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pubmed:articleTitle |
Rapid, direct enzyme immunoassay of 11-keto-thromboxane B2 in urine, validated by immunoaffinity/gas chromatography-mass spectrometry.
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pubmed:affiliation |
Novo Nordisk, Immunochemical Department, Bagsvaerd, Denmark.
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pubmed:publicationType |
Journal Article
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