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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
34
|
| pubmed:dateCreated |
1994-1-4
|
| pubmed:databankReference | |
| pubmed:abstractText |
We have previously isolated cDNA clones for the gip17 gene encoding the cytosolic nucleoside diphosphate (NDP) kinase from Dictyostelium discoideum, and partial cDNAs for guk, a second member of the NDP kinase gene family (Wallet, V., Mutzel, R., Troll, H., Barzu, O., Wurster, B., Véron, M., and Lacombe, M. L. (1990) J. Natl. Cancer Inst. 80, 1199-1202). We now characterize genomic DNA clones for both NDP kinase genes, and we show that guk defines a nuclear-encoded mitochondrial NDP kinase. Isolated D. discoideum mitochondria contain 3% of the total cellular NDP kinase activity. Antibodies which specifically recognize and inhibit the activity of either cytosolic or mitochondrial NDP kinase unambiguously distinguish between these activities. The nascent mitochondrial NDP kinase contains a presequence of 57 amino acids that is removed during import into the organelle as shown by determination of the NH2 terminus of the mature protein from mitochondria. The genes for mitochondrial and cytosolic NDP kinases contain four and two introns, respectively. The positions of the of the introns in the gene for the cytosolic enzyme match exactly the positions of the second and fourth introns in the coding region of its mitochondrial homologue. From these results we conclude that the isozymes diverged from a common ancestor, and we discuss possible phylogenetic pathways for the evolution of cytosolic and organelle NDP kinases.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Fungal,
http://linkedlifedata.com/resource/pubmed/chemical/Isoenzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Nucleoside-Diphosphate Kinase,
http://linkedlifedata.com/resource/pubmed/chemical/Oligodeoxyribonucleotides
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0021-9258
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
5
|
| pubmed:volume |
268
|
| pubmed:geneSymbol |
gip17,
guk,
nm23-H1
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
25469-75
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8244981-Amino Acid Sequence,
pubmed-meshheading:8244981-Animals,
pubmed-meshheading:8244981-Antibodies,
pubmed-meshheading:8244981-Base Sequence,
pubmed-meshheading:8244981-Cell Nucleus,
pubmed-meshheading:8244981-Cytosol,
pubmed-meshheading:8244981-DNA, Fungal,
pubmed-meshheading:8244981-Dictyostelium,
pubmed-meshheading:8244981-Exons,
pubmed-meshheading:8244981-Genes, Fungal,
pubmed-meshheading:8244981-Genomic Library,
pubmed-meshheading:8244981-Humans,
pubmed-meshheading:8244981-Introns,
pubmed-meshheading:8244981-Isoenzymes,
pubmed-meshheading:8244981-Mitochondria,
pubmed-meshheading:8244981-Molecular Sequence Data,
pubmed-meshheading:8244981-Nucleoside-Diphosphate Kinase,
pubmed-meshheading:8244981-Oligodeoxyribonucleotides,
pubmed-meshheading:8244981-Phylogeny,
pubmed-meshheading:8244981-Sequence Homology, Amino Acid
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
Separate nuclear genes encode cytosolic and mitochondrial nucleoside diphosphate kinase in Dictyostelium discoideum.
|
| pubmed:affiliation |
Fakultät für Biologie, Universität Konstanz, Federal Republic of Germany.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|