Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1994-1-5
pubmed:abstractText
The Staphylococcus aureus plasmid pT181 possesses a DNA replication enhancer element, called cmp, that is required in cis for optimal utilization of the initiator protein by the origin of replication. The minimal nucleotide sequence required for cmp activity was defined by testing progressively smaller DNA fragments for their ability to restore cmp activity in a plasmid mutant deleted for cmp. These experiments indicate that cmp is a sequence of 100 base pairs (bp) characterized by a loosely repeated sequence motif and phased oligo(dT) tracts. Intrinsic DNA bending at cmp was detected by a circular permutation assay of the locus using polyacrylamide-gel electrophoresis and by computer modeling. The cmp element was found to contain two loci of intrinsically bent DNA that confer an overall bent conformation to this replication enhancer.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0378-1119
pubmed:author
pubmed:issnType
Print
pubmed:day
30
pubmed:volume
134
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
93-8
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Sequence and structure of cmp, the replication enhancer of the Staphylococcus aureus plasmid pT181.
pubmed:affiliation
Public Health Research Institute, New York, NY 10016.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.