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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
9
|
| pubmed:dateCreated |
1993-11-26
|
| pubmed:abstractText |
Particulate wear debris from bone cement or prosthetic components can stimulate macrophages to cause bone resorption in a dose-dependent manner. This bone resorption activity of particulate-stimulated macrophages is associated with increased levels of both prostaglandin E2 (PGE2) and interleukin-1 (IL-1). In this study we compared the effect of particulate size, concentration, and composition on the secretion of IL-1 and PGE2 by peritoneal macrophages and on the bone-resorbing activity of conditioned medium (CM) harvested from particulate-challenged macrophages. Particulates (titanium, Ti; polymethylmethacrylate, PMMA; and polystyrene, PS) only with phagocytosable size stimulated peritoneal macrophages to secrete IL-1 and PGE2 in a dose- and time-dependent manner. Ti particles (1-3 microns) exhibited significantly enhanced bone-resorbing activity measured as 45Ca release. The maximum bone-resorbing response was observed at a concentration of 0.1% Ti (approximately 10-15 Ti particulates per cell), which also corresponded with the highest IL-1 levels measured in particulate-challenged CM. This was measured using either conditioned media from Ti-stimulated macrophages or in cocultures of calvarial bone and macrophages in the presence of Ti. Exogenous PGE2 and recombinant human IL-1 could significantly increase the 45Ca release; indomethacin (IM) significantly reduced both the spontaneous calcium efflux and active 45Ca release from in vivo labeled calvarial bones. However, IM and/or anti-IL-1 antibodies could suppress only partly the macrophage-mediated bone resorption, indicating that, in a macrophage-bone coculture system, factors other than PGE2 and IL-1 also may regulate particulate-induced bone resorption, probably involving multiple cell types.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media, Conditioned,
http://linkedlifedata.com/resource/pubmed/chemical/Dinoprostone,
http://linkedlifedata.com/resource/pubmed/chemical/Indomethacin,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-1,
http://linkedlifedata.com/resource/pubmed/chemical/Methylmethacrylates,
http://linkedlifedata.com/resource/pubmed/chemical/Polystyrenes,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Titanium
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0884-0431
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
8
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1071-9
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8237476-Animals,
pubmed-meshheading:8237476-Bone Resorption,
pubmed-meshheading:8237476-Calcium,
pubmed-meshheading:8237476-Cells, Cultured,
pubmed-meshheading:8237476-Culture Media, Conditioned,
pubmed-meshheading:8237476-Dinoprostone,
pubmed-meshheading:8237476-Female,
pubmed-meshheading:8237476-Indomethacin,
pubmed-meshheading:8237476-Interleukin-1,
pubmed-meshheading:8237476-Macrophages, Peritoneal,
pubmed-meshheading:8237476-Methylmethacrylates,
pubmed-meshheading:8237476-Mice,
pubmed-meshheading:8237476-Mice, Inbred BALB C,
pubmed-meshheading:8237476-Organ Culture Techniques,
pubmed-meshheading:8237476-Particle Size,
pubmed-meshheading:8237476-Phagocytosis,
pubmed-meshheading:8237476-Polystyrenes,
pubmed-meshheading:8237476-Recombinant Proteins,
pubmed-meshheading:8237476-Titanium
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
Bone resorption activity of particulate-stimulated macrophages.
|
| pubmed:affiliation |
Department of Biochemistry, Rush Medical College at Rush-Presbyterian, St. Luke's Medical Center, Chicago, Illinois.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|