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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
19
|
| pubmed:dateCreated |
1976-12-3
|
| pubmed:abstractText |
Wild type Bacillus subtilis, when grown on a soybean arabinan-galactan, secretes a beta-1,4-galactanase which has been purified more than 200-fold from the culture fluid. Affinity chromatography was the most effective step in a purification procedure which resulted in a preparation that contained only a single 40,000 molecular weight protein band upon sodium dodecyl sulfate-disc gel electrophoresis. The purified galactanase digests a beta-1,4-galactan purified from citrus pectin and digests partially the isolated cell walls of suspension-cultured sycamore cells. The predominant product of the enzymic degradation of the substrates tested is a 4-linked tetragalactose. Evidence is presented to support the hypothesis that the galactanase attacks its substrates in both an exo- and endo-manner. The products obtained upon galactanase digestion of the soybean arabianin-galactan demonstrate that the earlier proposal concerning the structure of this polysaccharide must be incorrect.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0021-9258
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
10
|
| pubmed:volume |
251
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
5904-10
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading | |
| pubmed:year |
1976
|
| pubmed:articleTitle |
Structure of plant cell walls. Purification and characterization of a beta-1,4-galactanase which degrades a structural component of the primary cell walls of dicots.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
|