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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
1993-12-21
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pubmed:abstractText |
The Oct-3 gene is expressed in highly undifferentiated cells and is implicated in mammalian early embryogenesis. We have generated a series of hybrid cells between pluripotent embryonal carcinoma cells (Oct-3+) and fibroblasts (Oct-3-), and have studied the regulation and function of Oct-3. Upon fusion, the hybrid cells differentiated to nestin+/Brn-2+ cells resembling neuroepithelial stem cells. Expression of Oct-3 was extinguished at the transcriptional level in all the hybrid cells examined. The Oct-3 modulating activity required for the Oct-3-mediated enhancer activation was also extinguished. When the Oct-3 transactivating function was introduced into the hybrid cells, they transformed into morphologically distinct nestin-/Brn-2- cells ('revertants'). When the 'revertant' cells subsequently lost Oct-3 expression, they differentiated back to nestin+/Brn-2+ cells. The close correlation between the phenotypic changes and the gain/loss of Oct-3 function indicates that Oct-3 can induce dedifferentiation of the neural cells.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8223459-1565620,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8223459-1689217,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8223459-1690859,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8223459-1830243,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8223459-1886774,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8223459-1915274,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8223459-1967980,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8223459-1972777,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8223459-2196176,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8223459-2216722,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8223459-2357966,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8223459-2536195,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8223459-2739723,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8223459-281705,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8223459-2960835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8223459-3047590,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8223459-3211142,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8223459-3215510,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8223459-3357014,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8223459-3670292,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8223459-4613257,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8223459-6310586,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8223459-6656766,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8223459-6960240,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8223459-7678695
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0261-4189
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
12
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pubmed:geneSymbol |
Oct-3
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4489-98
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8223459-Animals,
pubmed-meshheading:8223459-Base Sequence,
pubmed-meshheading:8223459-Blotting, Northern,
pubmed-meshheading:8223459-Cell Differentiation,
pubmed-meshheading:8223459-DNA,
pubmed-meshheading:8223459-DNA-Binding Proteins,
pubmed-meshheading:8223459-Embryonal Carcinoma Stem Cells,
pubmed-meshheading:8223459-Hybrid Cells,
pubmed-meshheading:8223459-L Cells (Cell Line),
pubmed-meshheading:8223459-Mice,
pubmed-meshheading:8223459-Molecular Sequence Data,
pubmed-meshheading:8223459-Neoplastic Stem Cells,
pubmed-meshheading:8223459-Octamer Transcription Factor-3,
pubmed-meshheading:8223459-Phenotype,
pubmed-meshheading:8223459-Transcription Factors,
pubmed-meshheading:8223459-Transcriptional Activation
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pubmed:year |
1993
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pubmed:articleTitle |
Hybrid cell extinction and re-expression of Oct-3 function correlates with differentiation potential.
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pubmed:affiliation |
Department of Biochemistry, Faculty of Medicine, University of Tokyo, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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