| pubmed-article:8215351 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8215351 | lifeskim:mentions | umls-concept:C0376325 | lld:lifeskim |
| pubmed-article:8215351 | lifeskim:mentions | umls-concept:C1511790 | lld:lifeskim |
| pubmed-article:8215351 | lifeskim:mentions | umls-concept:C0032520 | lld:lifeskim |
| pubmed-article:8215351 | lifeskim:mentions | umls-concept:C0035380 | lld:lifeskim |
| pubmed-article:8215351 | lifeskim:mentions | umls-concept:C1948027 | lld:lifeskim |
| pubmed-article:8215351 | lifeskim:mentions | umls-concept:C0998166 | lld:lifeskim |
| pubmed-article:8215351 | pubmed:issue | 9 | lld:pubmed |
| pubmed-article:8215351 | pubmed:dateCreated | 1993-11-10 | lld:pubmed |
| pubmed-article:8215351 | pubmed:abstractText | Hepatitis A virus (HAV) is a major cause of infectious hepatitis in humans. In this respect, bivalve mollusks pose a major health concern because they are filter feeders and can concentrate the virus up to 900-fold from contaminated water. Detection of HAV has been hampered because wild-type HAV grows poorly if at all in cell culture. Here we describe a technique for the detection of HAV in shellfish based on reverse transcription coupled with the polymerase chain reaction. RNA is isolated from hard-shell clam tissue and reverse transcribed with avian myeloblastosis virus reverse transcriptase. A portion of the cDNA pool is then amplified with primers specific for HAV. In experiments with an in vitro-synthesized HAV transcript, we were able to detect HAV sequence in the presence of a 200-million-fold excess of shellfish RNA. When intact virus was added to shellfish tissue before the isolation of RNA, the method was capable of detecting 10 viral RNA molecules in a reaction mixture. | lld:pubmed |
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| pubmed-article:8215351 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8215351 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8215351 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8215351 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:8215351 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8215351 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:8215351 | pubmed:issn | 0099-2240 | lld:pubmed |
| pubmed-article:8215351 | pubmed:author | pubmed-author:CebulaT ATA | lld:pubmed |
| pubmed-article:8215351 | pubmed:author | pubmed-author:GoswamiB BBB | lld:pubmed |
| pubmed-article:8215351 | pubmed:author | pubmed-author:KochW HWH | lld:pubmed |
| pubmed-article:8215351 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8215351 | pubmed:volume | 59 | lld:pubmed |
| pubmed-article:8215351 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8215351 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8215351 | pubmed:pagination | 2765-70 | lld:pubmed |
| pubmed-article:8215351 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:8215351 | pubmed:year | 1993 | lld:pubmed |
| pubmed-article:8215351 | pubmed:articleTitle | Detection of hepatitis A virus in Mercenaria mercenaria by coupled reverse transcription and polymerase chain reaction. | lld:pubmed |
| pubmed-article:8215351 | pubmed:affiliation | Division of Molecular Biological Research and Evaluation, Food and Drug Administration, Washington, D.C. 20204. | lld:pubmed |
| pubmed-article:8215351 | pubmed:publicationType | Journal Article | lld:pubmed |
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