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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
1993-11-10
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pubmed:abstractText |
Hepatitis A virus (HAV) is a major cause of infectious hepatitis in humans. In this respect, bivalve mollusks pose a major health concern because they are filter feeders and can concentrate the virus up to 900-fold from contaminated water. Detection of HAV has been hampered because wild-type HAV grows poorly if at all in cell culture. Here we describe a technique for the detection of HAV in shellfish based on reverse transcription coupled with the polymerase chain reaction. RNA is isolated from hard-shell clam tissue and reverse transcribed with avian myeloblastosis virus reverse transcriptase. A portion of the cDNA pool is then amplified with primers specific for HAV. In experiments with an in vitro-synthesized HAV transcript, we were able to detect HAV sequence in the presence of a 200-million-fold excess of shellfish RNA. When intact virus was added to shellfish tissue before the isolation of RNA, the method was capable of detecting 10 viral RNA molecules in a reaction mixture.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8215351-1318940,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8215351-1660697,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8215351-1714296,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8215351-1910780,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8215351-1928524,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8215351-1979752,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8215351-2157735,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8215351-2158093,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8215351-2413356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8215351-2447830,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8215351-2481313,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8215351-2829721,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8215351-3007565,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8215351-3022645,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8215351-3023706,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8215351-3657574,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8215351-6306048
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0099-2240
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
59
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2765-70
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8215351-Animals,
pubmed-meshheading:8215351-Base Sequence,
pubmed-meshheading:8215351-Bivalvia,
pubmed-meshheading:8215351-DNA, Viral,
pubmed-meshheading:8215351-DNA Primers,
pubmed-meshheading:8215351-Food Microbiology,
pubmed-meshheading:8215351-Hepatitis A,
pubmed-meshheading:8215351-Hepatovirus,
pubmed-meshheading:8215351-Humans,
pubmed-meshheading:8215351-Molecular Sequence Data,
pubmed-meshheading:8215351-Polymerase Chain Reaction,
pubmed-meshheading:8215351-RNA, Viral,
pubmed-meshheading:8215351-Sensitivity and Specificity,
pubmed-meshheading:8215351-Transcription, Genetic
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pubmed:year |
1993
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pubmed:articleTitle |
Detection of hepatitis A virus in Mercenaria mercenaria by coupled reverse transcription and polymerase chain reaction.
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pubmed:affiliation |
Division of Molecular Biological Research and Evaluation, Food and Drug Administration, Washington, D.C. 20204.
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pubmed:publicationType |
Journal Article
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