Linked Life Data

8214585

Source:http://linkedlifedata.com/resource/pubmed/id/8214585

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1993-11-16
pubmed:abstractText
Collagen extracted from tissues by pepsin digestion is a mixture of monomeric and oligomeric molecules. The oligomers are held together by covalent crosslinks between molecules. A simple size-exclusion high-performance liquid chromatography (HPLC) method for separation of collagen monomers and oligomers using a Bio-Gel TSK 60XL column has been developed for type I collagen from calf skin. Factors influencing the resolution include flow rate, the protein concentration of the sample, and the injection volume. To overcome electrostatic interactions between the protein and the column packing, addition of sodium chloride to the mobile phase was required to recover protein from the HPLC column when using 5 mM acetic acid as the mobile phase. Optimum recovery and oligomer content were obtained at 0.25 M NaCl. Component peaks eluting from the column were identified as monomers and oligomers by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Application of the method showed that increasing the oligomer content of collagen preparations accelerated fibrillogenesis in vitro and decreased the ultimate fibril size produced. Also during fibrillogenesis, collagen oligomers were preferentially incorporated into fibrils, leaving only monomeric collagen in the soluble supernatant fraction. The assay was also shown to be useful for measurement of conversion of monomers to oligomers during accelerated aging of collagen fibrils at 30 degrees C in vitro.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0003-2697
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
212
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
436-45
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Analysis of native collagen monomers and oligomers by size-exclusion high-performance liquid chromatography and its application.
pubmed:affiliation
Collagen Corporation, Palo Alto, California 94303.
pubmed:publicationType
Journal Article