Linked Life Data

8203491

Source:http://linkedlifedata.com/resource/pubmed/id/8203491

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5 Pt 1
pubmed:dateCreated
1994-7-7
pubmed:abstractText
The NADPH oxidase is one of the main microbicidal systems of granulocytes. Stimulation of the oxidase during infection leads to a burst of metabolic acid generation. Potentially deleterious cytosolic acidification is prevented by the simultaneous activation of homeostatic H+ extrusion mechanisms, including a recently described H+ conductance. Studies in granulocytes from chronic granulomatous disease patients have suggested a relationship between the oxidase and the H+ conductive pathway. In this report we compared the expression of the H+ conductance and the NADPH oxidase during granulocytic differentiation of dimethyl sulfoxide-induced HL-60 cells. Patch-clamp determinations demonstrated that the H(+)-selective current detectable in differentiated HL-60 cells is virtually absent in uninduced cells. The H+ conductance was also estimated fluorimetrically, measuring changes in the cytosolic pH of suspended cells. Imposition of an inward protonmotive force failed to induce significant cytosolic acidification. In contrast, a sizable conductive H+ extrusion was detected in acid-loaded differentiated cells, consistent with the rectifying properties of the current measured electrophysiologically. By the spectroscopic method, the H+ conductance was not detectable in uninduced cells, developing gradually during granulocytic differentiation. Development of the conductive pathway was found to parallel the biochemical and functional appearance of the NADPH oxidase. These findings suggest that the H+ extrusion mechanisms required for the maintenance of the intracellular pH during granulocyte activation develop pari passu with the acid generating systems and suggest a functional and possibly structural association between the H+ conductance and the NADPH oxidase.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0002-9513
pubmed:author
pubmed:issnType
Print
pubmed:volume
266
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
C1263-70
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:8203491-Amino Acid Sequence, pubmed-meshheading:8203491-Antibodies, pubmed-meshheading:8203491-Cell Differentiation, pubmed-meshheading:8203491-Cell Line, pubmed-meshheading:8203491-Dimethyl Sulfoxide, pubmed-meshheading:8203491-Electric Conductivity, pubmed-meshheading:8203491-Fluoresceins, pubmed-meshheading:8203491-Fluorescent Dyes, pubmed-meshheading:8203491-Gene Expression, pubmed-meshheading:8203491-Humans, pubmed-meshheading:8203491-Hydrogen-Ion Concentration, pubmed-meshheading:8203491-Immunoblotting, pubmed-meshheading:8203491-Leukemia, Myeloid, Acute, pubmed-meshheading:8203491-Molecular Sequence Data, pubmed-meshheading:8203491-Molecular Weight, pubmed-meshheading:8203491-NADH, NADPH Oxidoreductases, pubmed-meshheading:8203491-NADPH Oxidase, pubmed-meshheading:8203491-Peptides, pubmed-meshheading:8203491-Spectrometry, Fluorescence, pubmed-meshheading:8203491-Tetradecanoylphorbol Acetate, pubmed-meshheading:8203491-Tumor Cells, Cultured
pubmed:year
1994
pubmed:articleTitle
Development of a H(+)-selective conductance during granulocytic differentiation of HL-60 cells.
pubmed:affiliation
Division of Cell Biology, Hospital for Sick Children, Toronto, Ontario, Canada.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't