Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1994-7-5
|
| pubmed:databankReference | |
| pubmed:abstractText |
Embryogenesis-stimulating activity (ESA) was found in serum-free conditioned media (CM) of bovine cumulus/granulosa cells (BGC) and bovine oviductal epithelial cells (BOEC). The CM of BGC (BGC-CM) contained two molecular species of ESA, one with a low molecular weight (M(r) 30,000) and another with a high molecular weight (M(r) 80,000); but only the activity with low molecular weight was detected in CM of BOEC by gel-permeation chromatography. The smaller ESA (embryogenin-1) in BGC-CM was purified to homogeneity, as a common activity in both CM by a combination of gel-permeation chromatography, ion-exchange chromatography, and reverse-phase HPLC. Embryogenin-1 has a molecular weight of 31,100 (reduced) and has been identified as a bovine tissue inhibitor of metalloproteinase-1 by NH2-terminal amino acid sequence analysis. Western blot analysis, anti-proteinase activities against metalloproteinases, and the nucleotide sequence of cDNA isolated from a lambda gt11 cDNA library of the bovine ovary by a polyclonal antibody against embryogenin-1. These data suggest that the tissue inhibitor of metalloproteinase-1 produced by BGC and BOEC is a major ESA for in vitro development of bovine embryos.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media, Conditioned,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Metalloendopeptidases,
http://linkedlifedata.com/resource/pubmed/chemical/Tissue Inhibitor of...
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
0006-3363
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
50
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
835-44
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8199264-Amino Acid Sequence,
pubmed-meshheading:8199264-Animals,
pubmed-meshheading:8199264-Base Sequence,
pubmed-meshheading:8199264-Cattle,
pubmed-meshheading:8199264-Chromatography, Gel,
pubmed-meshheading:8199264-Cloning, Molecular,
pubmed-meshheading:8199264-Culture Media, Conditioned,
pubmed-meshheading:8199264-Culture Techniques,
pubmed-meshheading:8199264-DNA, Complementary,
pubmed-meshheading:8199264-Embryonic and Fetal Development,
pubmed-meshheading:8199264-Fallopian Tubes,
pubmed-meshheading:8199264-Female,
pubmed-meshheading:8199264-Glycoproteins,
pubmed-meshheading:8199264-Granulosa Cells,
pubmed-meshheading:8199264-Immunoblotting,
pubmed-meshheading:8199264-Metalloendopeptidases,
pubmed-meshheading:8199264-Molecular Sequence Data,
pubmed-meshheading:8199264-Molecular Weight,
pubmed-meshheading:8199264-Sequence Homology,
pubmed-meshheading:8199264-Tissue Inhibitor of Metalloproteinases
|
| pubmed:year |
1994
|
| pubmed:articleTitle |
Tissue inhibitor of metalloproteinases (TIMP-1) produced by granulosa and oviduct cells enhances in vitro development of bovine embryo.
|
| pubmed:affiliation |
Research Institute for the Functional Peptides, Yamagata, Japan.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|