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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
1994-6-27
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pubmed:abstractText |
DRAP27, the monkey homolog of human CD9 antigen (DRAP27/CD9) and diphtheria toxin receptor (DTR) were expressed in mouse L cells. L cells transfected transiently with both DRAP27/CD9 and DTR cDNA bound approximately 10 times more diphtheria toxin (DT) than cells transfected with DTR alone. Stable L cell transfectants expressing both DTR and DRAP27/CD9 (LCH-1 cells) had 15 times more cell surface DT-binding sites and were 20 times more sensitive to DT than were stable L cell transfectants expressing DTR alone (LH-1 cells). Increased DT-binding and DT sensitivity were not due to increased DTR transcription or increased cell surface DTR protein. Co-immunoprecipitation of DRAP27/CD9 with DTR and chemical cross-linking suggest a tight association of these membrane-bound proteins. In addition, the identity of DTR and a growth factor (HB-EGF) was established. Immobilized DT specifically adsorbed HB-EGF precursor solubilized from transfected L cells and [125I]DT bound to immobilized recombinant HB-EGF. We conclude that DRAP27/CD9 associates tightly with DTR/HB-EGF and up-regulates the number of functional DTRs and DT sensitivity, and that HB-EGF is identical to DTR.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8194524-1522113,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8194524-1556128,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8194524-1606612,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8194524-164179,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8194524-1840698,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8194524-1939100,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8194524-1939101,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8194524-194160,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8194524-1990012,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8194524-20040,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8194524-2147689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8194524-2254298,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8194524-2440339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8194524-2862151,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8194524-2925676,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8194524-3257584,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8194524-3273409,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8194524-3339019,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8194524-3417759,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8194524-3498987,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8194524-3654609,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8194524-3862242,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8194524-4044590,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8194524-4074708,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8194524-4196584,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8194524-6187735,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8194524-63947,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8194524-65205,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8194524-701254,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8194524-7159442,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8194524-7462324,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8194524-7678488,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8194524-8300582,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8194524-8349739,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8194524-8367482
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD9,
http://linkedlifedata.com/resource/pubmed/chemical/CD9 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Cd9 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Diphtheria Toxin,
http://linkedlifedata.com/resource/pubmed/chemical/Epidermal Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/heparin-binding EGF-like growth...
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0261-4189
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
13
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2322-30
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:8194524-Amino Acid Sequence,
pubmed-meshheading:8194524-Animals,
pubmed-meshheading:8194524-Antigens, CD,
pubmed-meshheading:8194524-Antigens, CD9,
pubmed-meshheading:8194524-Diphtheria Toxin,
pubmed-meshheading:8194524-Dose-Response Relationship, Drug,
pubmed-meshheading:8194524-Epidermal Growth Factor,
pubmed-meshheading:8194524-Humans,
pubmed-meshheading:8194524-Intercellular Signaling Peptides and Proteins,
pubmed-meshheading:8194524-L Cells (Cell Line),
pubmed-meshheading:8194524-Membrane Glycoproteins,
pubmed-meshheading:8194524-Membrane Proteins,
pubmed-meshheading:8194524-Mice,
pubmed-meshheading:8194524-Molecular Sequence Data,
pubmed-meshheading:8194524-Protein Binding,
pubmed-meshheading:8194524-Receptors, Cell Surface,
pubmed-meshheading:8194524-Recombinant Proteins,
pubmed-meshheading:8194524-Transfection,
pubmed-meshheading:8194524-Up-Regulation
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pubmed:year |
1994
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