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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1994-6-23
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pubmed:abstractText |
1. We describe the cloning of the inward rectifier K+ channel IRK1 from genomic DNA of mouse; the gene is intronless. 2. The IRK1 gene can be stably expressed in murine erythroleukaemia (MEL) cells. Such transfected cells show inward rectification under whole-cell recording. 3. Channels encoded by the IRK1 gene have an intrinsic gating that depends on voltage and [K+]o. Rate constants are reduced e-fold as the driving force on K+(V-EK) is reduced by 24.1 mV. 4. Removal of intracellular Mg2+ permits brief outward currents under depolarization. The instantaneous current-voltage relation may be fitted by an appropriate constant field expression. 5. Removal of intracellular Mg2+ speeds channel closure at positive voltages. In nominally zero [Mg2+]i, rate constants for the opening and closing of channels, processes which are first order, are similar to those of native channels.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8189383-1438587,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8189383-1547332,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8189383-2014181,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8189383-2433601,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8189383-2436236,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8189383-2457698,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8189383-2621633,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8189383-573790,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8189383-6694911,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8189383-6976432,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8189383-731684,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8189383-7680431,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8189383-7680768,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8189383-8081711,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8189383-8355805,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8189383-8450880
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0022-3751
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
475
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pubmed:geneSymbol |
IRK1
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1-7
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8189383-Animals,
pubmed-meshheading:8189383-Base Sequence,
pubmed-meshheading:8189383-Cells, Cultured,
pubmed-meshheading:8189383-Cloning, Molecular,
pubmed-meshheading:8189383-Electrophysiology,
pubmed-meshheading:8189383-Genome,
pubmed-meshheading:8189383-Introns,
pubmed-meshheading:8189383-Ion Channel Gating,
pubmed-meshheading:8189383-Leukemia, Erythroblastic, Acute,
pubmed-meshheading:8189383-Magnesium,
pubmed-meshheading:8189383-Mice,
pubmed-meshheading:8189383-Molecular Sequence Data,
pubmed-meshheading:8189383-Polymerase Chain Reaction,
pubmed-meshheading:8189383-Potassium,
pubmed-meshheading:8189383-Potassium Channels
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pubmed:year |
1994
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pubmed:articleTitle |
The intrinsic gating of inward rectifier K+ channels expressed from the murine IRK1 gene depends on voltage, K+ and Mg2+.
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pubmed:affiliation |
Department of Cell Physiology and Pharmacology, University of Leicester.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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