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rdf:type |
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lifeskim:mentions |
umls-concept:C0005821,
umls-concept:C0010837,
umls-concept:C0015283,
umls-concept:C0033640,
umls-concept:C0085862,
umls-concept:C0086418,
umls-concept:C0439794,
umls-concept:C0441712,
umls-concept:C1299583,
umls-concept:C1549571,
umls-concept:C1608386
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pubmed:issue |
1
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pubmed:dateCreated |
1994-6-23
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pubmed:abstractText |
We examined the mechanisms of ATP release by human platelets using Ro-31-7549, a specific inhibitor of protein kinase C. Ro-31-7549 almost completely inhibited TPA-induced platelet aggregation and ATP release at 5-10 microM in washed platelets and in platelet-rich plasma. However, it suppressed thrombin- and U46619-induced ATP release by only 48% and 21%, respectively, and had little effect on aggregation in washed platelet suspensions containing serum or in platelet-rich plasma. The addition of GRGDS to prevent aggregation inhibited this residual thrombin-induced release by 53% and the residual U46619 release by 100% in the presence of Ro-31-7549. In washed platelet suspensions free of serum or plasma, Ro-31-7549 almost completely inhibited the ATP release and partially suppressed the aggregation induced by these agonists. These results suggested that there are protein kinase C-dependent and -independent mechanisms for ATP release by human platelets and that activation of the latter mechanism may depend on aggregation and plasma factors.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/15-Hydroxy-11 alpha,9...,
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Indoles,
http://linkedlifedata.com/resource/pubmed/chemical/Maleimides,
http://linkedlifedata.com/resource/pubmed/chemical/Oligopeptides,
http://linkedlifedata.com/resource/pubmed/chemical/Platelet Aggregation Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Prostaglandin Endoperoxides...,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C,
http://linkedlifedata.com/resource/pubmed/chemical/Ro 31-7549,
http://linkedlifedata.com/resource/pubmed/chemical/Thrombin,
http://linkedlifedata.com/resource/pubmed/chemical/Thromboxane A2,
http://linkedlifedata.com/resource/pubmed/chemical/glycyl-arginyl-glycyl-aspartyl-serin...
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0006-3002
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
26
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pubmed:volume |
1222
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
56-62
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:8186265-15-Hydroxy-11 alpha,9...,
pubmed-meshheading:8186265-Adenosine Triphosphate,
pubmed-meshheading:8186265-Blood Platelets,
pubmed-meshheading:8186265-Cell Degranulation,
pubmed-meshheading:8186265-Enzyme Activation,
pubmed-meshheading:8186265-Exocytosis,
pubmed-meshheading:8186265-Humans,
pubmed-meshheading:8186265-Indoles,
pubmed-meshheading:8186265-Maleimides,
pubmed-meshheading:8186265-Oligopeptides,
pubmed-meshheading:8186265-Platelet Aggregation Inhibitors,
pubmed-meshheading:8186265-Prostaglandin Endoperoxides, Synthetic,
pubmed-meshheading:8186265-Protein Kinase C,
pubmed-meshheading:8186265-Thrombin,
pubmed-meshheading:8186265-Thromboxane A2
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pubmed:year |
1994
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pubmed:articleTitle |
Protein kinase C-dependent and -independent mechanisms of dense granule exocytosis by human platelets.
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pubmed:affiliation |
First Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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