Linked Life Data

8185623

Source:http://linkedlifedata.com/resource/pubmed/id/8185623

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1994-6-16
pubmed:databankReference
pubmed:abstractText
We have isolated the 5'flanking regions of two human Alpha class glutathione S-transferase genes, GSTA1 and GSTA2. The two genes share 95% sequence identity between nucleotide positions -1,300 and +500 from the transcriptional start site. Various DNA fragments from the 5' flanking region of the GSTA1 gene were fused to the chloramphenicol acetyltransferase reporter gene and transfected into HepG2 cells. The results indicated that negative regulatory and enhancer elements are located in the sequence upstream of the GSTA1 gene. Sequence analysis and functional assays have not found any evidence for xenobiotic- or antioxidant-responsive elements previously described in rodent Alpha class genes. Thus the transcriptional regulation of the human Alpha class glutathione S-transferase genes may be dramatically different from the regulation of Alpha class glutathione S-transferase genes in rodents.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0006-291X
pubmed:author
pubmed:issnType
Print
pubmed:day
16
pubmed:volume
200
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1665-71
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Structure and function of the 5' flanking sequences of the human alpha class glutathione S-transferase genes.
pubmed:affiliation
Molecular Genetics Group, John Curtin School of Medical Research, Australian National University, Canberra.
pubmed:publicationType
Journal Article, Comparative Study