Linked Life Data

8185571

Source:http://linkedlifedata.com/resource/pubmed/id/8185571

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1994-6-16
pubmed:abstractText
Total protein from N. tabacum cell cytosol, partially purified by ammonium sulphate precipitation, contains a 52 kDa protein that tests positive for mineralocorticoid receptor (MCR)-like activity by Western blots, immuneprecipitation and photochemical cross-linking. Binding of RU 26752, a ligand specific to the MCR, suggests 100 fmol steroid bound per mg of tobacco protein; this is equivalent to about 6000 sites per cell assuming a 1/1 stoichiometry. Northern blots of total RNA from isolated tobacco cells, hybridized with a probe specific to mammalian MCR, revealed 2.8 kb and 2.2 kb transcripts, whereas the poly A mRNA was resolved as a prominent message of 1500 bp, preceded by a band of 2200 bp. This appears to be the first ever demonstration where a vascular plant is endowed with a pygmy protein similar to the much larger steroid hormone receptors in the animal world.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0006-291X
pubmed:author
pubmed:issnType
Print
pubmed:day
16
pubmed:volume
200
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1230-8
pubmed:dateRevised
2005-11-17
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Nicotiana tabacum contains a putative mineralocorticoid receptor.
pubmed:affiliation
Centre Universitaire des Cordeliers, Paris, France.
pubmed:publicationType
Journal Article