Linked Life Data

8180696

Source:http://linkedlifedata.com/resource/pubmed/id/8180696

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1994-6-14
pubmed:databankReference
pubmed:abstractText
From a partial Sau3AI library of Streptomyces coelicolor A3(2) DNA in pIJ916, two hybrid plasmids pGX1 and pGX2 were isolated that complemented S. coelicolor A3(2) or S. lividans arginine auxotrophs. Subcloning DNA from pGX1 in the Escherichia coli expression vector pRK9 containing the Serratia marcescens trp promoter gave rise to one plasmid, pZC2, that complemented E. coli argB, C, E and H auxotrophs, and another, pZC1, that complemented only the first three. The plasmids were markedly unstable in the various complemented hosts, to varying extents; pZC1 was characterized further as providing the stablest host/plasmid combinations. In vitro deletion of part of the vector's trp promoter did not affect complementation of the argB and C auxotrophs, implying that the S. coelicolor A3(2) arg genes may be expressed from their own promoter. The trp promoter-less plasmids included isolates, such as pZC177, that had suffered extensive further deletion without loss of complementing ability. Extracts of an E. coli argE auxotroph carrying pZC177 showed ornithine acetyltransferase activity, indicating that the complementing gene is of the argJ type. The complementation properties of in vitro deletion derivatives of pZC177 indicated the gene order argC-J-B. Part of argC and the upstream region were sequenced; an ORF was identified whose predicted product showed appreciable homology with the E. coli and Bacillus subtilis ArgC polypeptide. Upstream of this ORF a consensus-type promoter and ribosome binding site could be discerned; overlapping its promoter was a sequence with homology to arginine operators in these two other organisms.(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1350-0872
pubmed:author
pubmed:issnType
Print
pubmed:volume
140 ( Pt 2)
pubmed:geneSymbol
argA, argB, argC, argE, argG, argH, argJ
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
311-20
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:8180696-Acetyltransferases, pubmed-meshheading:8180696-Aldehyde Oxidoreductases, pubmed-meshheading:8180696-Amino Acid Sequence, pubmed-meshheading:8180696-Amino-Acid N-Acetyltransferase, pubmed-meshheading:8180696-Bacterial Proteins, pubmed-meshheading:8180696-Base Sequence, pubmed-meshheading:8180696-Cloning, Molecular, pubmed-meshheading:8180696-DNA, Bacterial, pubmed-meshheading:8180696-Escherichia coli, pubmed-meshheading:8180696-Gene Expression Regulation, Bacterial, pubmed-meshheading:8180696-Genes, Bacterial, pubmed-meshheading:8180696-Genetic Complementation Test, pubmed-meshheading:8180696-Genetic Vectors, pubmed-meshheading:8180696-Molecular Sequence Data, pubmed-meshheading:8180696-Multigene Family, pubmed-meshheading:8180696-Plasmids, pubmed-meshheading:8180696-Promoter Regions, Genetic, pubmed-meshheading:8180696-Recombinant Fusion Proteins, pubmed-meshheading:8180696-Streptomyces, pubmed-meshheading:8180696-Transcription, Genetic
pubmed:year
1994
pubmed:articleTitle
Cloning and expression in Escherichia coli of a Streptomyces coelicolor A3(2) argCJB gene cluster.
pubmed:affiliation
Department of Genetics, University of Leeds, UK.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't