Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1994-6-1
pubmed:databankReference
pubmed:abstractText
The chromosomal region encoding the acidic class III chitinase from cucumber has been isolated and characterized. As a result of an apparent gene triplication, the pathogen-induced gene (CHI2) is flanked by two closely related genes with complete open reading frames (ORF). The high level of conservation within the three ORFs suggests an essential role for each encoded protein in plant growth and development. The developmental and tissue-specific expression of RNA from each gene was analyzed using both gene-specific probes and RNA-PCR. The expression of each gene in response to various inducing treatments was also characterized. Only transcripts corresponding to CHI2 were detected. Chitinase mRNA abundance increased slightly following cycloheximide application; however, its potent induction by salicylic acid was inhibited by cycloheximide treatment.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0894-0282
pubmed:author
pubmed:issnType
Print
pubmed:volume
7
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
48-57
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:articleTitle
Regulation of cucumber class III chitinase gene expression.
pubmed:affiliation
Department of Molecular Genetics, Ciba Agricultural Biotechnology, Research Triangle Park, NC 27709.
pubmed:publicationType
Journal Article, Comparative Study