Linked Life Data

8150261

Source:http://linkedlifedata.com/resource/pubmed/id/8150261

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1994-5-9
pubmed:databankReference
pubmed:abstractText
Caulobacters are biofilm bacteria that attach to surfaces via a holdfast, an adhesive expressed at discrete cell surface sites. We have described a cluster of at least three genes involved in the adhesive attachment of the holdfast of Caulobacter crescentus CB2A to the cell, analyzing the sequence of two genes, hfaAB. Here we report hfaC and a fourth open reading frame, hfaD. hfaC predicts a protein of 41 kDa homologous to ATP-binding transport-related proteins, with ChvD of Agrobacterium tumefaciens as best match. HfaD is predicted to be 28 kDa with three membrane spanning regions. hfaA, hfaC, and hfaD were expressed in Escherichia coli; Western analysis with antisera against a holdfast-enriched preparation indicated HfaA was likely holdfast-associated. Cumulative findings predict HfaA and HfaB are developmentally regulated and one or both enhance hfaC transcription, HfaA is a mediator of adhesion, possibly between holdfast and a membrane-bound HfaD, and HfaC mediates export of an unidentified component required for holdfast attachment.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0378-1097
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
116
pubmed:geneSymbol
hfaC, hfaD
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
175-82
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
The Caulobacter crescentus holdfast: identification of holdfast attachment complex genes.
pubmed:affiliation
Department of Microbiology, University of British Columbia, Vancouver, Canada.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't