|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:dateCreated |
1994-5-3
|
|
pubmed:abstractText |
1. Whole-cell and single-channel currents were recorded together with intracellular Ca2+ in voltage clamped, single endothelial cells isolated from human umbilical vein. 2. The major current component under resting conditions in the whole-cell configuration was a strongly inwardly rectifying potassium current. 3. This current is due to activation of a K+ channel with an inward conductance of 29 +/- 3 pS (n = 7) with symmetrical 140 mM K+ on both sides of the membrane. This channel could be measured both in the cell-attached and in the inside-out configuration. At potentials below -110 mV both whole-cell and averaged single-channel currents showed a fast inactivation. 4. During stimulation of endothelial cells with histamine, whole-cell K+ currents initially increased but then substantially declined, despite the sustained increase in intracellular Ca2+ concentration ([Ca2+]i). 5. The blockade of the inwardly rectifying K+ channel by histamine could not be observed in cell-attached patches if histamine was added to the bath. 6. It is concluded that endothelial cells possess K+ channels that are directly inhibited by agonists, such as histamine. Blocking these channels may depolarize the cell membrane and thereby reduce the driving force for Ca2+ influx.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8145150-1279867,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8145150-1329977,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8145150-1338792,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8145150-1415508,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8145150-1437527,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8145150-1484364,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8145150-1694153,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8145150-1700363,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8145150-1733236,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8145150-1770455,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8145150-1774774,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8145150-1895239,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8145150-2122922,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8145150-2145773,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8145150-2212977,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8145150-2381766,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8145150-2385008,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8145150-2388254,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8145150-2444358,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8145150-2444709,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8145150-2541447,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8145150-3177686,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8145150-3194169,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8145150-3838314,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8145150-6270629
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Dec
|
|
pubmed:issn |
0022-3751
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
472
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
359-71
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:8145150-Calcium,
pubmed-meshheading:8145150-Cells, Cultured,
pubmed-meshheading:8145150-Endothelium, Vascular,
pubmed-meshheading:8145150-Histamine,
pubmed-meshheading:8145150-Humans,
pubmed-meshheading:8145150-Intracellular Fluid,
pubmed-meshheading:8145150-Ion Transport,
pubmed-meshheading:8145150-Membrane Potentials,
pubmed-meshheading:8145150-Potassium Channel Blockers,
pubmed-meshheading:8145150-Potassium Channels
|
|
pubmed:year |
1993
|
|
pubmed:articleTitle |
Modulation by histamine of an inwardly rectifying potassium channel in human endothelial cells.
|
|
pubmed:affiliation |
Department of Physiology, KU Leuven, Belgium.
|
|
pubmed:publicationType |
Journal Article
|
