| pubmed-article:8144515 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8144515 | lifeskim:mentions | umls-concept:C0086045 | lld:lifeskim |
| pubmed-article:8144515 | lifeskim:mentions | umls-concept:C1511790 | lld:lifeskim |
| pubmed-article:8144515 | lifeskim:mentions | umls-concept:C1522602 | lld:lifeskim |
| pubmed-article:8144515 | lifeskim:mentions | umls-concept:C0392747 | lld:lifeskim |
| pubmed-article:8144515 | lifeskim:mentions | umls-concept:C0596235 | lld:lifeskim |
| pubmed-article:8144515 | lifeskim:mentions | umls-concept:C0679622 | lld:lifeskim |
| pubmed-article:8144515 | lifeskim:mentions | umls-concept:C0443172 | lld:lifeskim |
| pubmed-article:8144515 | lifeskim:mentions | umls-concept:C0205314 | lld:lifeskim |
| pubmed-article:8144515 | pubmed:issue | 13 | lld:pubmed |
| pubmed-article:8144515 | pubmed:dateCreated | 1994-5-5 | lld:pubmed |
| pubmed-article:8144515 | pubmed:abstractText | A Ca2+ indicator has been synthesized and characterized which can be used to monitor rapid changes in the free Ca2+ concentration ([Ca2+]) immediately adjacent to cell membranes. This indicator, referred to as C18-Fura-2, consists of a Fura-2 molecule conjugated to a lipophilic alkyl chain which will insert into cell membranes. When associated with cell membranes in low concentrations, C18-Fura-2 exhibits an excitation spectrum with a large Stokes shift and a single isobestic point, thus [Ca2+] can be calculated ratiometrically. The apparent Ca2+ dissociation constant of cell-associated C18-Fura-2 is around 150 nM. C18-Fura-2 orients in the cell membrane so that the fluorophore is facing the side to which it was applied. C18-Fura-2 was used to record rapid changes in intracellular [Ca2+] which occurred in response to membrane depolarization in isolated smooth muscle cells. The initial rise of the [Ca2+] transient reported by C18-Fura-2 was four to six times faster than the rise of the [Ca2+] transient reported by cytosolic Fura-2. This result suggests that C18-Fura-2 was located at the plasma membrane near sites of Ca2+ influx and indicates that membrane-associated Ca2+ indicators can be used to detect rapid, localized changes in [Ca2+] which are obscured in signals recorded using water-soluble, bulk cytosolic fluorescent Ca2+ indicators. | lld:pubmed |
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| pubmed-article:8144515 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8144515 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8144515 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8144515 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:8144515 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8144515 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:8144515 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:8144515 | pubmed:author | pubmed-author:KRAS JSJ | lld:pubmed |
| pubmed-article:8144515 | pubmed:author | pubmed-author:KuhnM AMA | lld:pubmed |
| pubmed-article:8144515 | pubmed:author | pubmed-author:EtterE FEF | lld:pubmed |
| pubmed-article:8144515 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8144515 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:8144515 | pubmed:volume | 269 | lld:pubmed |
| pubmed-article:8144515 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8144515 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8144515 | pubmed:pagination | 10141-9 | lld:pubmed |
| pubmed-article:8144515 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
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| pubmed-article:8144515 | pubmed:year | 1994 | lld:pubmed |
| pubmed-article:8144515 | pubmed:articleTitle | Detection of changes in near-membrane Ca2+ concentration using a novel membrane-associated Ca2+ indicator. | lld:pubmed |
| pubmed-article:8144515 | pubmed:affiliation | Department of Physiology, University of Massachusetts Medical School, Worcester 01605. | lld:pubmed |
| pubmed-article:8144515 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8144515 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:8144515 | pubmed:publicationType | In Vitro | lld:pubmed |
| pubmed-article:8144515 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:8144515 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
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