Linked Life Data

81306

Source:http://linkedlifedata.com/resource/pubmed/id/81306

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1978-12-27
pubmed:abstractText
The K+-stimulated, ouabain-insensitive ATPase activity present in vesicles of microsomal fractions from hog gastric mucosa can be demonstrated in fresh preparations by adding Ca2+ (micron range) to the incubation medium. Ca2+ effect is similar but not additive to the effect of gramicidin or freezing. High Ca2+ concentrations (1 mM) produce an inhibitory effect on the K+-stimulated ATPase activity. This effect is not seen in the presence of gramicidin. Calcium increases the magnitude of ATP-driven H+ uptake in vesicles exposed to K+ for periods of time up to 60 min. At longer times of exposure (120 min) the response does not differ from controls. It is concluded that Ca2+ at low concentrations (micron range) enhances the K+ permeability of the vesicular membrane. At higher concentrations (mM range), Ca2+ becomes inhibitory to the K+ permeability. A role for Ca2+ as a second messenger in stimulus-secretion coupling in the parietal cell is discussed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0022-2631
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
42
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
301-15
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1978
pubmed:articleTitle
Effect of calcium on the H+/K+ ATPase of hog gastric microsomes.
pubmed:publicationType
Journal Article, In Vitro