Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1994-4-11
pubmed:databankReference
pubmed:abstractText
Transcription factor IIIC (TFIIIC) is a multisubunit basic TF for RNA polymerase III. It initiates transcription complex assembly on tRNA and related genes by binding to the internal box B promoter element and is also required for transcription of 5S rRNA and other stable nuclear and cytoplasmic RNAs transcribed by polymerase III. In mammalian cells, regulation of TFIIIC activity controls overall polymerase III transcription in response to growth factors and viral infection. Here, we report the cloning and sequencing of a full-length cDNA (and genomic DNA from the transcription initiation region) encoding the box B binding subunit of human TFIIIC, the 243-kDa alpha subunit. Specific antisera raised against the encoded protein super shifts a TFIIIC-box B DNA complex during an electrophoretic mobility shift assay and immunodepletes TFIIIC transcriptional activity from a partially purified TFIIIC fraction, proving that the cDNA encodes a component of TFIIIC. The human protein shows surprisingly little similarity to the box B binding subunit of yeast TFIIIC.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8127861-1279682, http://linkedlifedata.com/resource/pubmed/commentcorrection/8127861-1398071, http://linkedlifedata.com/resource/pubmed/commentcorrection/8127861-14003696, http://linkedlifedata.com/resource/pubmed/commentcorrection/8127861-1423589, http://linkedlifedata.com/resource/pubmed/commentcorrection/8127861-1423590, http://linkedlifedata.com/resource/pubmed/commentcorrection/8127861-1447193, http://linkedlifedata.com/resource/pubmed/commentcorrection/8127861-1458536, http://linkedlifedata.com/resource/pubmed/commentcorrection/8127861-1497919, http://linkedlifedata.com/resource/pubmed/commentcorrection/8127861-1661258, http://linkedlifedata.com/resource/pubmed/commentcorrection/8127861-1737784, http://linkedlifedata.com/resource/pubmed/commentcorrection/8127861-2052571, http://linkedlifedata.com/resource/pubmed/commentcorrection/8127861-2100996, http://linkedlifedata.com/resource/pubmed/commentcorrection/8127861-2331366, http://linkedlifedata.com/resource/pubmed/commentcorrection/8127861-2482423, http://linkedlifedata.com/resource/pubmed/commentcorrection/8127861-2567002, http://linkedlifedata.com/resource/pubmed/commentcorrection/8127861-2732244, http://linkedlifedata.com/resource/pubmed/commentcorrection/8127861-2828918, http://linkedlifedata.com/resource/pubmed/commentcorrection/8127861-2940084, http://linkedlifedata.com/resource/pubmed/commentcorrection/8127861-2968157, http://linkedlifedata.com/resource/pubmed/commentcorrection/8127861-2987823, http://linkedlifedata.com/resource/pubmed/commentcorrection/8127861-2996135, http://linkedlifedata.com/resource/pubmed/commentcorrection/8127861-3052292, http://linkedlifedata.com/resource/pubmed/commentcorrection/8127861-3145406, http://linkedlifedata.com/resource/pubmed/commentcorrection/8127861-3243435, http://linkedlifedata.com/resource/pubmed/commentcorrection/8127861-3313383, http://linkedlifedata.com/resource/pubmed/commentcorrection/8127861-3473469, http://linkedlifedata.com/resource/pubmed/commentcorrection/8127861-3540951, http://linkedlifedata.com/resource/pubmed/commentcorrection/8127861-3597384, http://linkedlifedata.com/resource/pubmed/commentcorrection/8127861-3653074, http://linkedlifedata.com/resource/pubmed/commentcorrection/8127861-3667626, http://linkedlifedata.com/resource/pubmed/commentcorrection/8127861-3697084, http://linkedlifedata.com/resource/pubmed/commentcorrection/8127861-4005953, http://linkedlifedata.com/resource/pubmed/commentcorrection/8127861-6828386, http://linkedlifedata.com/resource/pubmed/commentcorrection/8127861-8387209, http://linkedlifedata.com/resource/pubmed/commentcorrection/8127861-8444147
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
91
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1652-6
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Human transcription factor IIIC box B binding subunit.
pubmed:affiliation
Department of Microbiology and Molecular Genetics, University of California, Los Angeles 90024-1570.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't