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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1994-4-11
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pubmed:abstractText |
In this study we have investigated whether cytokines, critical mediators of the immune response, might have a direct effect on the expression and/or function of the human hepatic asialoglycoprotein receptor (ASGPR). Binding and uptake of asialoglycoproteins by the human hepatoma cell line, HepG2, and by freshly isolated rat hepatocytes were inhibited by 50% after 3-6 hours and completely abolished following a 24 hour exposure to tumor necrosis factor (TNF) alpha, interferon (INF) alpha or gamma, or interleukin-2 (IL-2). The loss of ASGPR binding activity mediated by IL-2 was reversible up to 4 hours of exposure and accompanied by the selective phosphorylation of the cell-surface receptor. Steady-state levels of total cellular ASGPR protein remained unchanged over the first 6 hours of IL-2 incubation but declined in a dose dependent manner thereafter. This down regulation of ASGPR expression was due to reduced synthesis as a result of reduced receptor transcript levels. No loss was detected, however, of cell surface-associated receptor protein even after 24 hours of IL-2 incubation, suggesting that cytokine induced phosphorylation constitutes a mechanism to regulate receptor activity.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Asialoglycoprotein Receptor,
http://linkedlifedata.com/resource/pubmed/chemical/Asialoglycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-alpha,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-2,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-2,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0021-9541
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
158
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
527-34
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:8126076-Antibody Formation,
pubmed-meshheading:8126076-Asialoglycoprotein Receptor,
pubmed-meshheading:8126076-Asialoglycoproteins,
pubmed-meshheading:8126076-Carcinoma, Hepatocellular,
pubmed-meshheading:8126076-Cytokines,
pubmed-meshheading:8126076-Down-Regulation,
pubmed-meshheading:8126076-Humans,
pubmed-meshheading:8126076-Interferon-alpha,
pubmed-meshheading:8126076-Interferon-gamma,
pubmed-meshheading:8126076-Interleukin-2,
pubmed-meshheading:8126076-Liver Neoplasms,
pubmed-meshheading:8126076-Phosphorylation,
pubmed-meshheading:8126076-Receptors, Cell Surface,
pubmed-meshheading:8126076-Receptors, Interleukin-2,
pubmed-meshheading:8126076-Time Factors,
pubmed-meshheading:8126076-Transcription, Genetic,
pubmed-meshheading:8126076-Tumor Cells, Cultured,
pubmed-meshheading:8126076-Tumor Necrosis Factor-alpha
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pubmed:year |
1994
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pubmed:articleTitle |
Effects of cytokines on synthesis and function of the hepatic asialoglycoprotein receptor.
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pubmed:affiliation |
Department of Medicine and Biochemistry, Albert Einstein College of Medicine, Marion Bessin Liver Research Center, Bronx, New York 10461.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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