Linked Life Data

8125952

Source:http://linkedlifedata.com/resource/pubmed/id/8125952

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1994-4-12
pubmed:databankReference
pubmed:abstractText
The flavohemoprotein (FHP) encoding gene of the strictly respiratory Gram-negative bacterium Alcaligenes eutrophus was isolated from a megaplasmid library by using FHP-specific antibodies and oligonucleotide probes based on the amino-terminal polypeptide sequence of FHP, determined previously (Zhu, H., and Riggs, A. F. (1992) Proc. Natl. Acad. Sci. U.S.A. 89, 5015-5019). The fhp gene codes for a monomeric polypeptide of 403 amino acids (M(r) 44,796) whose structure is highly homologous to the proteins of the two-domain flavohemoglobin family, comprising the hemoproteins from Escherichia coli and Saccharomyces cerevisiae. FHP consists of an amino-terminal-located O2-binding hemoglobin domain and a carboxyl-terminal-located redoxactive domain with potential binding sites for NAD(P)H and FAD. Two potential binding motifs for NARL and FNR upstream of fhp suggest a role of FHP in the anaerobic metabolism of A. eutrophus. Isogenic Fhp-negative mutants showed no significant delay in aerobic or anaerobic growth. Compared with the wild type, however, the mutant did not accumulate nitrous oxide during denitrification with nitrite as electron acceptor. This property was restored by complementation. The result suggests that FHP interacts directly or indirectly with the gas metabolism during denitrification in A. eutrophus.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
11
pubmed:volume
269
pubmed:geneSymbol
fhp
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
7349-54
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Primary sequence and evidence for a physiological function of the flavohemoprotein of Alcaligenes eutrophus.
pubmed:affiliation
Institut für Pflanzenphysiologie und Mikrobiologie, Freie Universität Berlin, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't