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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
1994-4-12
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pubmed:abstractText |
Synthetic peptides derived from influenza virus and human immunodeficiency virus were tested for their ability to promote the assembly of HLA-A2 and HLA-B51 molecules in T2 cell lysates. Specific assembly was detected by an enzyme-linked immunosorbent assay. The most significant HLA-A2 assembly was obtained in the presence of peptides known to be targets for HLA-A2-restricted cytotoxic T lymphocytes (influenza matrix M.58-66 and HIV Pol 476-484). Three of a batch of Nef peptides corresponding to epitopic regions for cytotoxic T lymphocytes, caused significant assembly of HLA-A2 (Nef 83-91, 137-145 and 144-153), but only at high concentrations (100 microM). As these peptides bound relatively weakly, it is unlikely that they are good candidates for HLA-A2-restricted CTL epitopes. Peptides matrix M.60-68, Nef 186-194, and Plasmodium falciparum sh.77-85 produced the most significant assembly of HLA-B51. These peptides have a dominant hydrophobic anchor residue (V, L. I) at position 9 that could occupy pocket "F". Our results also suggest that another hydrophobic residue (V, L) at position 3 or 4 may anchor to hydrophobic pocket "D" of HLA-B51. Proline at position 2 greatly increases HLA-B51 anchoring.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Protozoan,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Gene Products, nef,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-A2 Antigen,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-B Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-B51 Antigen,
http://linkedlifedata.com/resource/pubmed/chemical/Macromolecular Substances,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0014-2980
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
24
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
777-80
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:8125145-Amino Acid Sequence,
pubmed-meshheading:8125145-Animals,
pubmed-meshheading:8125145-Antigens, Protozoan,
pubmed-meshheading:8125145-Antigens, Viral,
pubmed-meshheading:8125145-Gene Products, nef,
pubmed-meshheading:8125145-HLA-A2 Antigen,
pubmed-meshheading:8125145-HLA-B Antigens,
pubmed-meshheading:8125145-HLA-B51 Antigen,
pubmed-meshheading:8125145-Influenza A virus,
pubmed-meshheading:8125145-Macromolecular Substances,
pubmed-meshheading:8125145-Molecular Sequence Data,
pubmed-meshheading:8125145-Peptides,
pubmed-meshheading:8125145-Plasmodium falciparum,
pubmed-meshheading:8125145-Protein Binding,
pubmed-meshheading:8125145-Structure-Activity Relationship
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pubmed:year |
1994
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pubmed:articleTitle |
A simple assay for detection of peptides promoting the assembly of HLA class I molecules.
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pubmed:affiliation |
Institut Cochin de Génétique Moléculaire (ICGM), INSERM U152, Paris, France.
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pubmed:publicationType |
Journal Article
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